Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32768 | 5' | -55.3 | NC_007346.1 | + | 71767 | 0.66 | 0.993884 |
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Target: 5'- aUCAGUUGUUGCaGCAGAaGUAccGUUGAUc -3' miRNA: 3'- -GGUCAGCGACG-CGUCUaCGU--CAGCUG- -5' |
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| 32768 | 5' | -55.3 | NC_007346.1 | + | 163177 | 0.66 | 0.993019 |
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Target: 5'- aCAGUCGCaGUGguGAUggGCGGU-GAUg -3' miRNA: 3'- gGUCAGCGaCGCguCUA--CGUCAgCUG- -5' |
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| 32768 | 5' | -55.3 | NC_007346.1 | + | 154163 | 0.66 | 0.990997 |
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Target: 5'- cCC--UCGCUGCgGCGGGcgucgggucUGCAGUCG-Ca -3' miRNA: 3'- -GGucAGCGACG-CGUCU---------ACGUCAGCuG- -5' |
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| 32768 | 5' | -55.3 | NC_007346.1 | + | 286098 | 0.67 | 0.983913 |
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Target: 5'- aCCAGUCGagguUGuCGguGuUGCAG-CGACu -3' miRNA: 3'- -GGUCAGCg---AC-GCguCuACGUCaGCUG- -5' |
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| 32768 | 5' | -55.3 | NC_007346.1 | + | 260253 | 0.68 | 0.970604 |
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Target: 5'- aCCgGGUCGUcgUGCGCGcAUGC-GUCGAUa -3' miRNA: 3'- -GG-UCAGCG--ACGCGUcUACGuCAGCUG- -5' |
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| 32768 | 5' | -55.3 | NC_007346.1 | + | 274094 | 0.69 | 0.958194 |
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Target: 5'- -aAGaCGCcGUGCGGAUGCGGU-GACg -3' miRNA: 3'- ggUCaGCGaCGCGUCUACGUCAgCUG- -5' |
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| 32768 | 5' | -55.3 | NC_007346.1 | + | 46798 | 0.7 | 0.938179 |
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Target: 5'- gCAGUUGCgguUGCGCGGAUGCaauuaauaacgGGUugUGACg -3' miRNA: 3'- gGUCAGCG---ACGCGUCUACG-----------UCA--GCUG- -5' |
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| 32768 | 5' | -55.3 | NC_007346.1 | + | 65763 | 0.71 | 0.895165 |
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Target: 5'- gUCGGUUGUugcagGCGguGAUGCGGUUGGa -3' miRNA: 3'- -GGUCAGCGa----CGCguCUACGUCAGCUg -5' |
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| 32768 | 5' | -55.3 | NC_007346.1 | + | 308752 | 0.71 | 0.895165 |
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Target: 5'- -gGGUCGUcgaUGCGUGGAUGCAaucUCGGCa -3' miRNA: 3'- ggUCAGCG---ACGCGUCUACGUc--AGCUG- -5' |
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| 32768 | 5' | -55.3 | NC_007346.1 | + | 87353 | 1.11 | 0.007272 |
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Target: 5'- cCCAGUCGCUGCGCAGAUGCAGUCGACc -3' miRNA: 3'- -GGUCAGCGACGCGUCUACGUCAGCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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