miRNA: 3'- -GCUGCUa----GUAGCA----AUGCUGCUGCUAg -5'
miRNA: 3'- -GCUGCUAGUAGCAauGC--UGCUGCUAG- -5'
miRNA: 3'- -GCUGCUAGUAGCaaUGc------UGCUGCUAG- -5'
miRNA: 3'- -GCUGCUAGUAGCAa-UGC-----UGCUGCUAG- -5'
miRNA: 3'- -GCUGCUAGUAGCAAUGCUGCUGCUAG- -5'
Results 41 - 45 of 45 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32771 | 5' | -48.1 | NC_007346.1 | + | 280784 | 0.75 | 0.986752 |
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Target: 5'- cCGACGGauaugCAUCGUgugaUACGGCGAUGGUg -3' miRNA: 3'- -GCUGCUa----GUAGCA----AUGCUGCUGCUAg -5' |
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| 32771 | 5' | -48.1 | NC_007346.1 | + | 109417 | 0.79 | 0.943957 |
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Target: 5'- aCGAUGAUCGUCGU--CGuaACGAUGAUCg -3' miRNA: 3'- -GCUGCUAGUAGCAauGC--UGCUGCUAG- -5' |
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| 32771 | 5' | -48.1 | NC_007346.1 | + | 109894 | 0.82 | 0.829792 |
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Target: 5'- aCGACGAUCAUCGcgACcccguccACGACGAUCa -3' miRNA: 3'- -GCUGCUAGUAGCaaUGc------UGCUGCUAG- -5' |
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| 32771 | 5' | -48.1 | NC_007346.1 | + | 109450 | 0.83 | 0.795895 |
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Target: 5'- aCGAUGAUCGUCGUggACGgggucGCGAUGAUCg -3' miRNA: 3'- -GCUGCUAGUAGCAa-UGC-----UGCUGCUAG- -5' |
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| 32771 | 5' | -48.1 | NC_007346.1 | + | 109855 | 1.13 | 0.033527 |
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Target: 5'- aCGACGAUCAUCGUUACGACGACGAUCa -3' miRNA: 3'- -GCUGCUAGUAGCAAUGCUGCUGCUAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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