Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32772 | 3' | -57.3 | NC_007346.1 | + | 305122 | 0.66 | 0.979945 |
Target: 5'- cACCCGgcuGGUCGCCuccaGCGUgaGCGUUGCa -3' miRNA: 3'- cUGGGCag-UCGGUGG----UGCA--CGUAGCG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 243870 | 0.66 | 0.979741 |
Target: 5'- -cCCCGUCgguuugcGGUCACuCAgGUGCAcgaguugaUCGCa -3' miRNA: 3'- cuGGGCAG-------UCGGUG-GUgCACGU--------AGCG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 128111 | 0.66 | 0.97784 |
Target: 5'- aACUCcaCcGCCGCCAUcUGCAUCGCc -3' miRNA: 3'- cUGGGcaGuCGGUGGUGcACGUAGCG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 39943 | 0.66 | 0.97784 |
Target: 5'- uGAuCCCGagGGCC-CCaaACGUGCAacaCGCg -3' miRNA: 3'- -CU-GGGCagUCGGuGG--UGCACGUa--GCG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 275427 | 0.66 | 0.974141 |
Target: 5'- cGCaCGUCAGCCGCCGuguacaccucugacuUGUGUgugaacagcguGUCGCa -3' miRNA: 3'- cUGgGCAGUCGGUGGU---------------GCACG-----------UAGCG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 189003 | 0.66 | 0.970552 |
Target: 5'- --gCCGUCAccGCCACCAC---CGUCGCc -3' miRNA: 3'- cugGGCAGU--CGGUGGUGcacGUAGCG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 264286 | 0.67 | 0.961689 |
Target: 5'- aGCUCGUCGuUCGCCGCGUGaAUCGa -3' miRNA: 3'- cUGGGCAGUcGGUGGUGCACgUAGCg -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 272047 | 0.67 | 0.958363 |
Target: 5'- cGCCaCcUCcGCCACCGCcgGUGCGUCGg -3' miRNA: 3'- cUGG-GcAGuCGGUGGUG--CACGUAGCg -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 306744 | 0.67 | 0.958363 |
Target: 5'- aGCCCGUCGccGCCGCCAaGUcCAcCGCc -3' miRNA: 3'- cUGGGCAGU--CGGUGGUgCAcGUaGCG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 303376 | 0.67 | 0.954844 |
Target: 5'- cAUCCGUUAGgaCACCAaGUGCAaugCGCg -3' miRNA: 3'- cUGGGCAGUCg-GUGGUgCACGUa--GCG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 135438 | 0.67 | 0.951128 |
Target: 5'- aAUCCGUCAGCUucGCCGauaaCAUCGCa -3' miRNA: 3'- cUGGGCAGUCGG--UGGUgcacGUAGCG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 156830 | 0.68 | 0.943097 |
Target: 5'- ---aUGUCAGUCGCCGCaguaUGCGUUGCg -3' miRNA: 3'- cuggGCAGUCGGUGGUGc---ACGUAGCG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 309310 | 0.68 | 0.943097 |
Target: 5'- aGCCCGUCGccGCCACCAaGUcCAcCGCc -3' miRNA: 3'- cUGGGCAGU--CGGUGGUgCAcGUaGCG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 39563 | 0.68 | 0.924586 |
Target: 5'- cGACCCGgCAGCCACU-CGgugGCuGUCGg -3' miRNA: 3'- -CUGGGCaGUCGGUGGuGCa--CG-UAGCg -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 89894 | 0.69 | 0.919443 |
Target: 5'- aGCaaGcCAGCUGCCGCGgaaGCAUUGCa -3' miRNA: 3'- cUGggCaGUCGGUGGUGCa--CGUAGCG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 165236 | 0.69 | 0.901014 |
Target: 5'- cAUUCGUCauguGGCCAaaaaauucacgcguCUGCGUGCGUCGCg -3' miRNA: 3'- cUGGGCAG----UCGGU--------------GGUGCACGUAGCG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 272891 | 0.7 | 0.871022 |
Target: 5'- cGCaCCGUCAGCgCAuCCAUGgaaaacacgaGCGUCGCg -3' miRNA: 3'- cUG-GGCAGUCG-GU-GGUGCa---------CGUAGCG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 370848 | 0.71 | 0.842269 |
Target: 5'- uGACuuGUCAaCUACCAUGUGCuugaggCGCu -3' miRNA: 3'- -CUGggCAGUcGGUGGUGCACGua----GCG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 315573 | 0.72 | 0.759747 |
Target: 5'- cGACCaUGUCAGCaucauCACCAUGUGCAgauGCa -3' miRNA: 3'- -CUGG-GCAGUCG-----GUGGUGCACGUag-CG- -5' |
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32772 | 3' | -57.3 | NC_007346.1 | + | 352679 | 0.73 | 0.727279 |
Target: 5'- cGCCCGauguaaaguUCAuGCCACCgggugccaucgauucACGUGCAUUGCg -3' miRNA: 3'- cUGGGC---------AGU-CGGUGG---------------UGCACGUAGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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