Results 1 - 17 of 17 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32772 | 5' | -55.1 | NC_007346.1 | + | 61126 | 0.66 | 0.990503 |
Target: 5'- gGCGGagGAGGUgGUGAUGGC---GCCGGu -3' miRNA: 3'- -UGCU--UUCCGgCGCUACCGauuCGGUU- -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 50243 | 0.66 | 0.988311 |
Target: 5'- gACGAGcaauGGCCGCGgcGGCggaacaucagaaaauGCCAGc -3' miRNA: 3'- -UGCUUu---CCGGCGCuaCCGauu------------CGGUU- -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 197749 | 0.66 | 0.987886 |
Target: 5'- gGCGAuggugguGGUgGCGAUGGCggugGAGgCGAu -3' miRNA: 3'- -UGCUuu-----CCGgCGCUACCGa---UUCgGUU- -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 200630 | 0.66 | 0.984224 |
Target: 5'- gGCGAugguguuguuggugGAGGCgGUGAUGGUgauacauuuGGCCAc -3' miRNA: 3'- -UGCU--------------UUCCGgCGCUACCGau-------UCGGUu -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 310254 | 0.66 | 0.982957 |
Target: 5'- gGCGGAuuuGGCgGCGGUGGUaAAGgCGg -3' miRNA: 3'- -UGCUUu--CCGgCGCUACCGaUUCgGUu -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 116382 | 0.67 | 0.976647 |
Target: 5'- cCGAGuAGGCUGCGGUGGUacAAGCg-- -3' miRNA: 3'- uGCUU-UCCGGCGCUACCGa-UUCGguu -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 87206 | 0.67 | 0.975692 |
Target: 5'- cGCGAuggaggagcuuauGGGCCGCGcgGGCc--GCCGu -3' miRNA: 3'- -UGCUu------------UCCGGCGCuaCCGauuCGGUu -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 349783 | 0.67 | 0.974205 |
Target: 5'- gGCGGc-GGCgGCGGUGGCgUGAGgCAGc -3' miRNA: 3'- -UGCUuuCCGgCGCUACCG-AUUCgGUU- -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 49996 | 0.67 | 0.974205 |
Target: 5'- gGCGAuggugguGGUgGCGAUGGCgAGGCgGAu -3' miRNA: 3'- -UGCUuu-----CCGgCGCUACCGaUUCGgUU- -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 165597 | 0.67 | 0.974205 |
Target: 5'- gACGAAGguGGCgGCGAUGGUgguGGCgAu -3' miRNA: 3'- -UGCUUU--CCGgCGCUACCGau-UCGgUu -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 128670 | 0.68 | 0.968772 |
Target: 5'- cCGAAcGGCgGCGAUGGUgAAGCa-- -3' miRNA: 3'- uGCUUuCCGgCGCUACCGaUUCGguu -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 165567 | 0.68 | 0.96257 |
Target: 5'- gGCGAAGguGGCgGCGAUGGCgaugguGGCg-- -3' miRNA: 3'- -UGCUUU--CCGgCGCUACCGau----UCGguu -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 57512 | 0.68 | 0.959168 |
Target: 5'- gGCGAggGAGGCgGCGAUGGa--GGCgGAc -3' miRNA: 3'- -UGCU--UUCCGgCGCUACCgauUCGgUU- -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 353575 | 0.69 | 0.947706 |
Target: 5'- uCGcAAGGCUG-GucUGGCUAAGCCAAu -3' miRNA: 3'- uGCuUUCCGGCgCu-ACCGAUUCGGUU- -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 134806 | 0.69 | 0.929386 |
Target: 5'- -aGAAAGGCgGCGAagaaGGCggcaaagAAGCCAGu -3' miRNA: 3'- ugCUUUCCGgCGCUa---CCGa------UUCGGUU- -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 188405 | 0.71 | 0.868226 |
Target: 5'- gGCG-GAGGCgGCGAUGGCggAGGCg-- -3' miRNA: 3'- -UGCuUUCCGgCGCUACCGa-UUCGguu -5' |
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32772 | 5' | -55.1 | NC_007346.1 | + | 110535 | 1.06 | 0.011969 |
Target: 5'- cACGAAAGGCCGCGAUGGCUAAGCCAAa -3' miRNA: 3'- -UGCUUUCCGGCGCUACCGAUUCGGUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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