Results 1 - 16 of 16 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32773 | 5' | -55.2 | NC_007346.1 | + | 349346 | 0.66 | 0.992635 |
Target: 5'- cCUGCUgCCUcacgCCACcgCCGCCGCc-- -3' miRNA: 3'- -GACGA-GGAca--GGUGaaGGUGGCGaac -5' |
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32773 | 5' | -55.2 | NC_007346.1 | + | 307041 | 0.67 | 0.984851 |
Target: 5'- cCUGCUCCaccaCCGCcuacUCCACCGCc-- -3' miRNA: 3'- -GACGAGGaca-GGUGa---AGGUGGCGaac -5' |
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32773 | 5' | -55.2 | NC_007346.1 | + | 307384 | 0.67 | 0.984851 |
Target: 5'- -cGCUCCaccUCCACccaaUCCACCGCc-- -3' miRNA: 3'- gaCGAGGac-AGGUGa---AGGUGGCGaac -5' |
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32773 | 5' | -55.2 | NC_007346.1 | + | 308455 | 0.67 | 0.984851 |
Target: 5'- cCUGCUCCaccaCCGCcuacUCCACCGCc-- -3' miRNA: 3'- -GACGAGGaca-GGUGa---AGGUGGCGaac -5' |
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32773 | 5' | -55.2 | NC_007346.1 | + | 101077 | 0.69 | 0.959519 |
Target: 5'- gUGCuUCCUGUCUuucagcuuuucguGCUUCCAUUGCa-- -3' miRNA: 3'- gACG-AGGACAGG-------------UGAAGGUGGCGaac -5' |
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32773 | 5' | -55.2 | NC_007346.1 | + | 185689 | 0.69 | 0.944556 |
Target: 5'- -cGUaCUUGUCCAUUUgCCGCCGCUc- -3' miRNA: 3'- gaCGaGGACAGGUGAA-GGUGGCGAac -5' |
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32773 | 5' | -55.2 | NC_007346.1 | + | 218332 | 0.71 | 0.903709 |
Target: 5'- aCUGCUCCacccCCACUUCCucuGCUGCUUc -3' miRNA: 3'- -GACGAGGaca-GGUGAAGG---UGGCGAAc -5' |
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32773 | 5' | -55.2 | NC_007346.1 | + | 57113 | 0.71 | 0.891359 |
Target: 5'- cCUGaUCCUGcUCCACcaCCACCGCcUGg -3' miRNA: 3'- -GACgAGGAC-AGGUGaaGGUGGCGaAC- -5' |
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32773 | 5' | -55.2 | NC_007346.1 | + | 307329 | 0.71 | 0.891359 |
Target: 5'- cCUGCUCCaccgCCAUcUCCACCGCc-- -3' miRNA: 3'- -GACGAGGaca-GGUGaAGGUGGCGaac -5' |
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32773 | 5' | -55.2 | NC_007346.1 | + | 307862 | 0.71 | 0.891359 |
Target: 5'- cCUGCUCCaccgCCAUcUCCACCGCc-- -3' miRNA: 3'- -GACGAGGaca-GGUGaAGGUGGCGaac -5' |
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32773 | 5' | -55.2 | NC_007346.1 | + | 308135 | 0.71 | 0.891359 |
Target: 5'- cCUGCUCCaccgCCAUcUCCACCGCc-- -3' miRNA: 3'- -GACGAGGaca-GGUGaAGGUGGCGaac -5' |
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32773 | 5' | -55.2 | NC_007346.1 | + | 307916 | 0.71 | 0.884873 |
Target: 5'- cCUGCUCCaccUCCACccaaUCCACCGCc-- -3' miRNA: 3'- -GACGAGGac-AGGUGa---AGGUGGCGaac -5' |
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32773 | 5' | -55.2 | NC_007346.1 | + | 308189 | 0.71 | 0.884873 |
Target: 5'- cCUGCUCCaccUCCACccaaUCCACCGCc-- -3' miRNA: 3'- -GACGAGGac-AGGUGa---AGGUGGCGaac -5' |
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32773 | 5' | -55.2 | NC_007346.1 | + | 306951 | 0.73 | 0.809667 |
Target: 5'- cCUGCUCCgccgCCAUcUCCACCGCc-- -3' miRNA: 3'- -GACGAGGaca-GGUGaAGGUGGCGaac -5' |
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32773 | 5' | -55.2 | NC_007346.1 | + | 296681 | 0.77 | 0.58476 |
Target: 5'- uUGCcCCUG-CCGCUUCCGCUGCUa- -3' miRNA: 3'- gACGaGGACaGGUGAAGGUGGCGAac -5' |
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32773 | 5' | -55.2 | NC_007346.1 | + | 156495 | 1.09 | 0.008978 |
Target: 5'- gCUGCUCCUGUCCACUUCCACCGCUUGa -3' miRNA: 3'- -GACGAGGACAGGUGAAGGUGGCGAAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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