Results 1 - 8 of 8 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32775 | 3' | -54.1 | NC_007346.1 | + | 99608 | 0.66 | 0.994739 |
Target: 5'- cUAAuCGUguGGGCAAGUGuuGCAa-- -3' miRNA: 3'- -GUUcGCAguCCCGUUUACggCGUgau -5' |
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32775 | 3' | -54.1 | NC_007346.1 | + | 133536 | 0.66 | 0.994739 |
Target: 5'- gAAGgGUCAGGGCAAGaGCgGUcUUGg -3' miRNA: 3'- gUUCgCAGUCCCGUUUaCGgCGuGAU- -5' |
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32775 | 3' | -54.1 | NC_007346.1 | + | 364569 | 0.67 | 0.986845 |
Target: 5'- aCGAGCGUCAGGcgaauCAAAUGCCaucuugaGCAUc- -3' miRNA: 3'- -GUUCGCAGUCCc----GUUUACGG-------CGUGau -5' |
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32775 | 3' | -54.1 | NC_007346.1 | + | 305133 | 0.68 | 0.979709 |
Target: 5'- -cAGCGUCGGuGGCGAGaauUGCgGCGgUGg -3' miRNA: 3'- guUCGCAGUC-CCGUUU---ACGgCGUgAU- -5' |
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32775 | 3' | -54.1 | NC_007346.1 | + | 147500 | 0.68 | 0.979709 |
Target: 5'- uCGuuCGUCAGuGGCAucgauUGCCGCACc- -3' miRNA: 3'- -GUucGCAGUC-CCGUuu---ACGGCGUGau -5' |
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32775 | 3' | -54.1 | NC_007346.1 | + | 177838 | 0.7 | 0.9356 |
Target: 5'- gCGGGUG-CGGGcGCAGGUGCgGCugUAg -3' miRNA: 3'- -GUUCGCaGUCC-CGUUUACGgCGugAU- -5' |
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32775 | 3' | -54.1 | NC_007346.1 | + | 283579 | 0.71 | 0.920261 |
Target: 5'- uGAGCGUCGcaaGGCAAAUGCgGCAa-- -3' miRNA: 3'- gUUCGCAGUc--CCGUUUACGgCGUgau -5' |
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32775 | 3' | -54.1 | NC_007346.1 | + | 160628 | 1.07 | 0.011905 |
Target: 5'- aCAAGCGUCAGGGCAAAUGCCGCACUAg -3' miRNA: 3'- -GUUCGCAGUCCCGUUUACGGCGUGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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