miRNA: 3'- cCGUUACGU-C-CU--GC-ACCGUGG-CCugC- -5'
miRNA: 3'- -CCGUUACGUC-CU--GCaCCG---------UGGCCUGC- -5'
miRNA: 3'- -CCGuUACGUCCugCA--------CCGUGG-C-CUGc -5'
Results 121 - 123 of 123 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 32775 | 5' | -56.7 | NC_007346.1 | + | 353137 | 0.67 | 0.97784 |
|
Target: 5'- cGCAAUGCAcGuGAauCGaUGGCACCcGGugGc -3' miRNA: 3'- cCGUUACGU-C-CU--GC-ACCGUGG-CCugC- -5' |
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| 32775 | 5' | -56.7 | NC_007346.1 | + | 361643 | 0.67 | 0.975577 |
|
Target: 5'- cGGUAAUGCGGaGAacCGcGGCgguccaugaACCGGAUGu -3' miRNA: 3'- -CCGUUACGUC-CU--GCaCCG---------UGGCCUGC- -5' |
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| 32775 | 5' | -56.7 | NC_007346.1 | + | 401578 | 0.7 | 0.902781 |
|
Target: 5'- uGGCcGUGCAGGugGUuguugagcGGCGCCcGcGGCu -3' miRNA: 3'- -CCGuUACGUCCugCA--------CCGUGG-C-CUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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