Results 1 - 15 of 15 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32776 | 3' | -53.3 | NC_007346.1 | + | 237720 | 0.66 | 0.998859 |
Target: 5'- uUCUugUACAuacugauCCCGgAUCGGGUUUAa- -3' miRNA: 3'- -AGAugAUGU-------GGGUgUGGCCCAGAUcu -5' |
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32776 | 3' | -53.3 | NC_007346.1 | + | 70691 | 0.66 | 0.998752 |
Target: 5'- cCUugUAUugCCAC-CCGGuacaaagggaagaccGUCUGGGu -3' miRNA: 3'- aGAugAUGugGGUGuGGCC---------------CAGAUCU- -5' |
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32776 | 3' | -53.3 | NC_007346.1 | + | 353305 | 0.66 | 0.998661 |
Target: 5'- --gAUUACACCCACGa-GGGUgUAGu -3' miRNA: 3'- agaUGAUGUGGGUGUggCCCAgAUCu -5' |
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32776 | 3' | -53.3 | NC_007346.1 | + | 214341 | 0.66 | 0.998661 |
Target: 5'- aCUGCcuuuguaaACGCCUcgaaGCACCGGGUUuugUGGAc -3' miRNA: 3'- aGAUGa-------UGUGGG----UGUGGCCCAG---AUCU- -5' |
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32776 | 3' | -53.3 | NC_007346.1 | + | 214637 | 0.66 | 0.998661 |
Target: 5'- aCUGCcuuuguaaACGCCUcgaaGCACCGGGUUuugUGGAc -3' miRNA: 3'- aGAUGa-------UGUGGG----UGUGGCCCAG---AUCU- -5' |
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32776 | 3' | -53.3 | NC_007346.1 | + | 179990 | 0.66 | 0.998407 |
Target: 5'- -gUGC-ACGCCCAUACUguGGGUC-GGAu -3' miRNA: 3'- agAUGaUGUGGGUGUGG--CCCAGaUCU- -5' |
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32776 | 3' | -53.3 | NC_007346.1 | + | 213950 | 0.66 | 0.997777 |
Target: 5'- cUUUGCaaACGCCUcgaaGCACCGGGUUuugUGGAc -3' miRNA: 3'- -AGAUGa-UGUGGG----UGUGGCCCAG---AUCU- -5' |
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32776 | 3' | -53.3 | NC_007346.1 | + | 214741 | 0.66 | 0.997777 |
Target: 5'- cUUUGCaaACGCCUcgaaGCACCGGGUUuugUGGAc -3' miRNA: 3'- -AGAUGa-UGUGGG----UGUGGCCCAG---AUCU- -5' |
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32776 | 3' | -53.3 | NC_007346.1 | + | 124450 | 0.67 | 0.99645 |
Target: 5'- gCUGCUGCgAUUgACGCCGGGUacaaAGAu -3' miRNA: 3'- aGAUGAUG-UGGgUGUGGCCCAga--UCU- -5' |
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32776 | 3' | -53.3 | NC_007346.1 | + | 29763 | 0.67 | 0.995179 |
Target: 5'- aCUaACUGCAaaauaaCCAUACUuguuuuuGGGUCUAGAu -3' miRNA: 3'- aGA-UGAUGUg-----GGUGUGG-------CCCAGAUCU- -5' |
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32776 | 3' | -53.3 | NC_007346.1 | + | 40008 | 0.68 | 0.990044 |
Target: 5'- gUCUAgUGCACCgACagccaccgaguggcuGCCGGGUCg--- -3' miRNA: 3'- -AGAUgAUGUGGgUG---------------UGGCCCAGaucu -5' |
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32776 | 3' | -53.3 | NC_007346.1 | + | 373219 | 0.72 | 0.945166 |
Target: 5'- uUCUGCUACGCCagcauCACCGGG---GGAa -3' miRNA: 3'- -AGAUGAUGUGGgu---GUGGCCCagaUCU- -5' |
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32776 | 3' | -53.3 | NC_007346.1 | + | 263842 | 0.72 | 0.945166 |
Target: 5'- -gUACaACACCCACACgGGGUgCUuGAa -3' miRNA: 3'- agAUGaUGUGGGUGUGgCCCA-GAuCU- -5' |
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32776 | 3' | -53.3 | NC_007346.1 | + | 64225 | 0.74 | 0.856871 |
Target: 5'- cUUAUUGCACCCGCACUccguGGGUCUucguauaAGAa -3' miRNA: 3'- aGAUGAUGUGGGUGUGG----CCCAGA-------UCU- -5' |
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32776 | 3' | -53.3 | NC_007346.1 | + | 165178 | 1.1 | 0.012779 |
Target: 5'- cUCUACUACACCCACACCGGGUCUAGAg -3' miRNA: 3'- -AGAUGAUGUGGGUGUGGCCCAGAUCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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