Results 1 - 20 of 21 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32776 | 5' | -57.8 | NC_007346.1 | + | 70966 | 0.66 | 0.975547 |
Target: 5'- uGCagCACCGAuaCCGAuGCGCGAGc--GCAu -3' miRNA: 3'- gCG--GUGGCU--GGCU-CGCGCUCuaaCGU- -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 228347 | 0.66 | 0.975547 |
Target: 5'- aCGCCGCCgggauuGACUGAGaCGCcgccGGGAUUGa- -3' miRNA: 3'- -GCGGUGG------CUGGCUC-GCG----CUCUAACgu -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 228284 | 0.66 | 0.967778 |
Target: 5'- aCGCCGCCgggauuGACUGAGaCGCcgccGGGAUUGgGu -3' miRNA: 3'- -GCGGUGG------CUGGCUC-GCG----CUCUAACgU- -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 208053 | 0.67 | 0.954902 |
Target: 5'- aCGCCguGCCGuucguGCCGAGCucguUGAGAUUGUu -3' miRNA: 3'- -GCGG--UGGC-----UGGCUCGc---GCUCUAACGu -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 50806 | 0.67 | 0.954902 |
Target: 5'- aCGCCAgUGAcaaCCGccauaGCGAGGUUGCAu -3' miRNA: 3'- -GCGGUgGCU---GGCucg--CGCUCUAACGU- -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 243103 | 0.67 | 0.947312 |
Target: 5'- -uCCGCCGACauuGAGCGCGcg--UGCAa -3' miRNA: 3'- gcGGUGGCUGg--CUCGCGCucuaACGU- -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 306387 | 0.67 | 0.947312 |
Target: 5'- uGCaGCCGugCG-GCGCGAGGgaacgGCu -3' miRNA: 3'- gCGgUGGCugGCuCGCGCUCUaa---CGu -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 86887 | 0.67 | 0.938483 |
Target: 5'- aCGuCCACCucgaggcGGCCGAGCGUGA---UGCGc -3' miRNA: 3'- -GC-GGUGG-------CUGGCUCGCGCUcuaACGU- -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 228389 | 0.68 | 0.919704 |
Target: 5'- aCGCCACCGAguuugaCUGAGaCGCcgucGAGAUUGa- -3' miRNA: 3'- -GCGGUGGCU------GGCUC-GCG----CUCUAACgu -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 308986 | 0.69 | 0.903146 |
Target: 5'- gCGCaGCCGugCG-GCGCGAGGgaacgGCu -3' miRNA: 3'- -GCGgUGGCugGCuCGCGCUCUaa---CGu -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 272055 | 0.69 | 0.88479 |
Target: 5'- cCGCCACCG-CCG-GUGCGucGGUccucUGCAa -3' miRNA: 3'- -GCGGUGGCuGGCuCGCGCu-CUA----ACGU- -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 258904 | 0.69 | 0.878285 |
Target: 5'- aCGCguCCGAgCGugGGuCGCGAGAUUGUg -3' miRNA: 3'- -GCGguGGCUgGC--UC-GCGCUCUAACGu -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 256791 | 0.71 | 0.811835 |
Target: 5'- uGCgACCGAgCGugGGuCGCGAGAUUGUg -3' miRNA: 3'- gCGgUGGCUgGC--UC-GCGCUCUAACGu -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 257529 | 0.71 | 0.811835 |
Target: 5'- uGCgACCGAgCGugGGuCGCGAGAUUGUg -3' miRNA: 3'- gCGgUGGCUgGC--UC-GCGCUCUAACGu -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 259969 | 0.71 | 0.811835 |
Target: 5'- uGCgACCGAgCGugGGuCGCGAGAUUGUg -3' miRNA: 3'- gCGgUGGCUgGC--UC-GCGCUCUAACGu -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 258130 | 0.75 | 0.621006 |
Target: 5'- gGCCGCUGcguCCGAGCguggcucGCGAGAUUGUg -3' miRNA: 3'- gCGGUGGCu--GGCUCG-------CGCUCUAACGu -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 292605 | 0.77 | 0.498757 |
Target: 5'- cCGCCACCGgggcagccauacccGCCGAGCGCGAuGUgGCGc -3' miRNA: 3'- -GCGGUGGC--------------UGGCUCGCGCUcUAaCGU- -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 254653 | 0.78 | 0.432463 |
Target: 5'- uCGCCGCCuucuCUGcGCGCGAGAUUGCGc -3' miRNA: 3'- -GCGGUGGcu--GGCuCGCGCUCUAACGU- -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 253543 | 0.81 | 0.299771 |
Target: 5'- gGCCACCuucuCCGcGCGCGAGAUUGCGc -3' miRNA: 3'- gCGGUGGcu--GGCuCGCGCUCUAACGU- -5' |
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32776 | 5' | -57.8 | NC_007346.1 | + | 242474 | 0.82 | 0.293399 |
Target: 5'- uGCCACCGccuucuCCGcGCGUGAGAUUGCAc -3' miRNA: 3'- gCGGUGGCu-----GGCuCGCGCUCUAACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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