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miRNA: 3'- gaAGCa---GCGGUGGUAGCGG---------UAGCGGc -5'
miRNA: 3'- -GAAGCAGCGGUGGuagCGGUAGcGGC- -5'
miRNA: 3'- gaaGCAGCGGUGgu------AGCGGUAGCGGc -5'
miRNA: 3'- gaagCAGc-GGUGGUAGCGGUAGCGGc -5'
miRNA: 3'- -GAAGCAGCGGUGGUAGCGGUAGCGGC- -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio#
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P value |
| Predicted miRNA align pattern | |||||||
| 32777 | 5' | -56.8 | NC_007346.1 | + | 198236 | 0.78 | 0.524743 |
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Target: 5'- -aUCGccacCGCCACCAUCGCCuccaccgccAUCGCCa -3' miRNA: 3'- gaAGCa---GCGGUGGUAGCGG---------UAGCGGc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 58498 | 0.78 | 0.515658 |
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Target: 5'- cCUUCGUCGCCACC---GCCGUCaCCGa -3' miRNA: 3'- -GAAGCAGCGGUGGuagCGGUAGcGGC- -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 50446 | 0.8 | 0.388859 |
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Target: 5'- --gCGUCGCCACaauccgccUCGCCAUCGCCa -3' miRNA: 3'- gaaGCAGCGGUGgu------AGCGGUAGCGGc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 127890 | 0.82 | 0.344165 |
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Target: 5'- ---aGUCcaCCACCAUCGCCAUCGCCa -3' miRNA: 3'- gaagCAGc-GGUGGUAGCGGUAGCGGc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 166010 | 1.08 | 0.00878 |
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Target: 5'- cCUUCGUCGCCACCAUCGCCAUCGCCGc -3' miRNA: 3'- -GAAGCAGCGGUGGUAGCGGUAGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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