Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
|
P value |
| Predicted miRNA align pattern | |||||||
| 32777 | 5' | -56.8 | NC_007346.1 | + | 274755 | 0.66 | 0.979233 |
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Target: 5'- --aUGUCuGCCGCCAcgcucagccgUCGugcCCGUCGCCa -3' miRNA: 3'- gaaGCAG-CGGUGGU----------AGC---GGUAGCGGc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 188953 | 0.66 | 0.981232 |
|
Target: 5'- -----cCGCCACCAUcacCGCCAccuccaUCGCCu -3' miRNA: 3'- gaagcaGCGGUGGUA---GCGGU------AGCGGc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 306803 | 0.66 | 0.981232 |
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Target: 5'- ----aUCGCCACCAcCaCCAUUGCCu -3' miRNA: 3'- gaagcAGCGGUGGUaGcGGUAGCGGc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 310026 | 0.66 | 0.981232 |
|
Target: 5'- --cCGUCaccacccCCACCAUcCGCC-UCGCCa -3' miRNA: 3'- gaaGCAGc------GGUGGUA-GCGGuAGCGGc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 323635 | 0.66 | 0.987814 |
|
Target: 5'- --gUGUaagUGUC-CCAaCGCCGUCGCCGa -3' miRNA: 3'- gaaGCA---GCGGuGGUaGCGGUAGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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