miRNA: 3'- gaAGCAG--------CggUGGUAGCGGUAGCGGC- -5'
miRNA: 3'- gaagcaGCGGUGGUAGc---------GGUAGCGGc -5'
miRNA: 3'- -GAAGCAGCGGUGGUa---GCGGU---AGcggc -5'
miRNA: 3'- gaagcaGCGGUGGUAGc---------GGUAGCGGc -5'
miRNA: 3'- gaaGCA---GCGGuGGUaGCGGUAGCGGC- -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32777 | 5' | -56.8 | NC_007346.1 | + | 179821 | 0.66 | 0.987814 |
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Target: 5'- -cUCGUCaauuucgaGagGCCAUCGUCAUUGCUGg -3' miRNA: 3'- gaAGCAG--------CggUGGUAGCGGUAGCGGC- -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 198574 | 0.66 | 0.987814 |
|
Target: 5'- -----cCGCCACCAUCacccccaccaCCAUCGCCc -3' miRNA: 3'- gaagcaGCGGUGGUAGc---------GGUAGCGGc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 38112 | 0.66 | 0.987814 |
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Target: 5'- uCUUCGUCGUCACUAgaugCGCCAauuUCa--- -3' miRNA: 3'- -GAAGCAGCGGUGGUa---GCGGU---AGcggc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 198742 | 0.66 | 0.987814 |
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Target: 5'- -----cCGCCACCAUCacccccaccaCCAUCGCCc -3' miRNA: 3'- gaagcaGCGGUGGUAGc---------GGUAGCGGc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 323635 | 0.66 | 0.987814 |
|
Target: 5'- --gUGUaagUGUC-CCAaCGCCGUCGCCGa -3' miRNA: 3'- gaaGCA---GCGGuGGUaGCGGUAGCGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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