miRNA: 3'- gaAGcaGCGGUGGUaGCGGUAGCGGC- -5'
miRNA: 3'- gaagcaGCGGUGGUaGCGGUaGCGGc -5'
miRNA: 3'- gAAGCAGcGGUGGUAGCGGU---AGCGgc -5'
miRNA: 3'- gaaGCAGCGGUGgu------AGCGGUAGCGGc -5'
miRNA: 3'- -GAAGCAGCGGUGGUa---GCGGU---AGcggc -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
|
R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 32777 | 5' | -56.8 | NC_007346.1 | + | 57885 | 0.67 | 0.974768 |
|
Target: 5'- -aUCucCGCCACCGcCGUUAUUGCCGc -3' miRNA: 3'- gaAGcaGCGGUGGUaGCGGUAGCGGC- -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 57182 | 0.66 | 0.984793 |
|
Target: 5'- -----cCGCCAUCAcCGCCAcCGCCa -3' miRNA: 3'- gaagcaGCGGUGGUaGCGGUaGCGGc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 55176 | 0.67 | 0.97708 |
|
Target: 5'- gUUCGUCaUCAUCAUCGCCAaacUUGCg- -3' miRNA: 3'- gAAGCAGcGGUGGUAGCGGU---AGCGgc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 50446 | 0.8 | 0.388859 |
|
Target: 5'- --gCGUCGCCACaauccgccUCGCCAUCGCCa -3' miRNA: 3'- gaaGCAGCGGUGgu------AGCGGUAGCGGc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 38112 | 0.66 | 0.987814 |
|
Target: 5'- uCUUCGUCGUCACUAgaugCGCCAauuUCa--- -3' miRNA: 3'- -GAAGCAGCGGUGGUa---GCGGU---AGcggc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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