miRNA: 3'- gaAGCA-------GCGGUGGUaGCGGUa---GCGGc -5'
miRNA: 3'- gaaGCAGcGGUGGUAGCGGUaGCGGc -5'
miRNA: 3'- gaAGCAGCGGUGGU---------------AGCGGuaGCGGC- -5'
miRNA: 3'- gaaGCaGcGGUGGUAGCGGUaGCGGc -5'
miRNA: 3'- gaagCAGc-GGUGGUAGCGGUAGCGGc -5'
Results 101 - 105 of 105 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 32777 | 5' | -56.8 | NC_007346.1 | + | 272017 | 0.7 | 0.900477 |
|
Target: 5'- -gUCGUguaccacCGCCACCAcCGCCAccucCGCCa -3' miRNA: 3'- gaAGCA-------GCGGUGGUaGCGGUa---GCGGc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 270225 | 0.7 | 0.895068 |
|
Target: 5'- --cCGUCaCCGCCGUUGCCAcCGUCa -3' miRNA: 3'- gaaGCAGcGGUGGUAGCGGUaGCGGc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 329920 | 0.71 | 0.87855 |
|
Target: 5'- -aUCGUCGUCAUCGuccgaaucgggaaguUCGUCcgCGCCGu -3' miRNA: 3'- gaAGCAGCGGUGGU---------------AGCGGuaGCGGC- -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 127806 | 0.71 | 0.875895 |
|
Target: 5'- --cCGcCaCCACCGUCGCCGcCGCCa -3' miRNA: 3'- gaaGCaGcGGUGGUAGCGGUaGCGGc -5' |
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| 32777 | 5' | -56.8 | NC_007346.1 | + | 127890 | 0.82 | 0.344165 |
|
Target: 5'- ---aGUCcaCCACCAUCGCCAUCGCCa -3' miRNA: 3'- gaagCAGc-GGUGGUAGCGGUAGCGGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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