Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32779 | 5' | -55.6 | NC_007346.1 | + | 261135 | 0.66 | 0.991171 |
Target: 5'- uGggCGACGCGCGUccgaguGUgGCuCGCGGGAu -3' miRNA: 3'- -CuaGCUGCGUGCA------CAgCGcGCGCUUU- -5' |
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32779 | 5' | -55.6 | NC_007346.1 | + | 257376 | 0.66 | 0.991171 |
Target: 5'- ---gGAUGCGCGUGUCaCGCGUa--- -3' miRNA: 3'- cuagCUGCGUGCACAGcGCGCGcuuu -5' |
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32779 | 5' | -55.6 | NC_007346.1 | + | 48415 | 0.66 | 0.985846 |
Target: 5'- --aCGAUGCACuUGUCGCGCaucGCGc-- -3' miRNA: 3'- cuaGCUGCGUGcACAGCGCG---CGCuuu -5' |
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32779 | 5' | -55.6 | NC_007346.1 | + | 253982 | 0.67 | 0.978353 |
Target: 5'- uGUCG-CGCAauc-UCGCGCGCGGAGa -3' miRNA: 3'- cUAGCuGCGUgcacAGCGCGCGCUUU- -5' |
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32779 | 5' | -55.6 | NC_007346.1 | + | 259489 | 0.67 | 0.97609 |
Target: 5'- --cCGACGaUugGauaUCGCGCGCGAGAu -3' miRNA: 3'- cuaGCUGC-GugCac-AGCGCGCGCUUU- -5' |
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32779 | 5' | -55.6 | NC_007346.1 | + | 280015 | 0.68 | 0.968267 |
Target: 5'- uGGUgGAUGCGacccUGcCGCGCGCGAAAc -3' miRNA: 3'- -CUAgCUGCGUgc--ACaGCGCGCGCUUU- -5' |
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32779 | 5' | -55.6 | NC_007346.1 | + | 241443 | 0.68 | 0.965296 |
Target: 5'- --cCGAgaGCAaCGcGUCGCGCGCGAu- -3' miRNA: 3'- cuaGCUg-CGU-GCaCAGCGCGCGCUuu -5' |
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32779 | 5' | -55.6 | NC_007346.1 | + | 274050 | 0.68 | 0.965296 |
Target: 5'- cAUCGGCGU---UGUCGCGCGCa--- -3' miRNA: 3'- cUAGCUGCGugcACAGCGCGCGcuuu -5' |
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32779 | 5' | -55.6 | NC_007346.1 | + | 259624 | 0.68 | 0.958779 |
Target: 5'- aGUCGAUGCACuUGUCGCaCGCa--- -3' miRNA: 3'- cUAGCUGCGUGcACAGCGcGCGcuuu -5' |
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32779 | 5' | -55.6 | NC_007346.1 | + | 250731 | 0.69 | 0.934363 |
Target: 5'- --gCGugGCGCGUGgCGCGUuCGAAAc -3' miRNA: 3'- cuaGCugCGUGCACaGCGCGcGCUUU- -5' |
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32779 | 5' | -55.6 | NC_007346.1 | + | 258752 | 0.73 | 0.808532 |
Target: 5'- aGUCGGCGUACGcaaucagcauggUGUCGCGaGCGAAGu -3' miRNA: 3'- cUAGCUGCGUGC------------ACAGCGCgCGCUUU- -5' |
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32779 | 5' | -55.6 | NC_007346.1 | + | 171356 | 1.06 | 0.013385 |
Target: 5'- uGAUCGACGCACGUGUCGCGCGCGAAAg -3' miRNA: 3'- -CUAGCUGCGUGCACAGCGCGCGCUUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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