Results 1 - 12 of 12 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32780 | 3' | -53.8 | NC_007346.1 | + | 154464 | 0.66 | 0.994365 |
Target: 5'- uAUGugGGCGGUUaCGCUUauCugUGCGg -3' miRNA: 3'- -UACugUCGUCGA-GCGAAacGugACGU- -5' |
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32780 | 3' | -53.8 | NC_007346.1 | + | 220620 | 0.66 | 0.994365 |
Target: 5'- -cGACuGCAGUUUGUaUUGCaucguugaGCUGCAu -3' miRNA: 3'- uaCUGuCGUCGAGCGaAACG--------UGACGU- -5' |
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32780 | 3' | -53.8 | NC_007346.1 | + | 156083 | 0.66 | 0.994365 |
Target: 5'- --aGCAGCAGCa-GCgggUGCuGCUGCAc -3' miRNA: 3'- uacUGUCGUCGagCGaa-ACG-UGACGU- -5' |
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32780 | 3' | -53.8 | NC_007346.1 | + | 54735 | 0.66 | 0.99352 |
Target: 5'- cAUGACAcGUuGCUUGCUgugUGCAUauagUGCAu -3' miRNA: 3'- -UACUGU-CGuCGAGCGAa--ACGUG----ACGU- -5' |
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32780 | 3' | -53.8 | NC_007346.1 | + | 44148 | 0.66 | 0.990361 |
Target: 5'- -gGACaAGCAGCggCGC---GCGCUGCu -3' miRNA: 3'- uaCUG-UCGUCGa-GCGaaaCGUGACGu -5' |
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32780 | 3' | -53.8 | NC_007346.1 | + | 401094 | 0.67 | 0.987659 |
Target: 5'- gAUGGCAGCuGCagGCg--GCGgUGCAc -3' miRNA: 3'- -UACUGUCGuCGagCGaaaCGUgACGU- -5' |
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32780 | 3' | -53.8 | NC_007346.1 | + | 303321 | 0.67 | 0.984406 |
Target: 5'- aAUG-CAGCGGCUCGUUgcugaGCAaUGCGa -3' miRNA: 3'- -UACuGUCGUCGAGCGAaa---CGUgACGU- -5' |
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32780 | 3' | -53.8 | NC_007346.1 | + | 275251 | 0.68 | 0.978356 |
Target: 5'- -cGACAGCGGCUCGgUaucaaUGaGCUGCu -3' miRNA: 3'- uaCUGUCGUCGAGCgAa----ACgUGACGu -5' |
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32780 | 3' | -53.8 | NC_007346.1 | + | 19501 | 0.68 | 0.970726 |
Target: 5'- cGUGAUuGCAGCgCGUUUuuuauugaagaaUGCACUGCu -3' miRNA: 3'- -UACUGuCGUCGaGCGAA------------ACGUGACGu -5' |
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32780 | 3' | -53.8 | NC_007346.1 | + | 137762 | 0.72 | 0.855487 |
Target: 5'- -aGAUAGCAGC-CGCaa-GUACUGCAa -3' miRNA: 3'- uaCUGUCGUCGaGCGaaaCGUGACGU- -5' |
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32780 | 3' | -53.8 | NC_007346.1 | + | 216908 | 0.77 | 0.633327 |
Target: 5'- --cAUAGCAacGCUCGCUUUGCAgUGCAa -3' miRNA: 3'- uacUGUCGU--CGAGCGAAACGUgACGU- -5' |
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32780 | 3' | -53.8 | NC_007346.1 | + | 217358 | 1.07 | 0.012192 |
Target: 5'- uAUGACAGCAGCUCGCUUUGCACUGCAa -3' miRNA: 3'- -UACUGUCGUCGAGCGAAACGUGACGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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