Results 1 - 9 of 9 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32780 | 5' | -47.6 | NC_007346.1 | + | 180028 | 0.66 | 0.999998 |
Target: 5'- aUUGgUGU----CGUACCAGACCAUGa -3' miRNA: 3'- cAACgAUAcuucGUAUGGUCUGGUAC- -5' |
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32780 | 5' | -47.6 | NC_007346.1 | + | 54723 | 0.67 | 0.999994 |
Target: 5'- cUUGCUGUGu-GCAUAU-AGugCAUGg -3' miRNA: 3'- cAACGAUACuuCGUAUGgUCugGUAC- -5' |
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32780 | 5' | -47.6 | NC_007346.1 | + | 384018 | 0.68 | 0.999977 |
Target: 5'- aUUGC-AUGggGUAcACCAGACUuUGu -3' miRNA: 3'- cAACGaUACuuCGUaUGGUCUGGuAC- -5' |
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32780 | 5' | -47.6 | NC_007346.1 | + | 372485 | 0.68 | 0.999977 |
Target: 5'- -cUGCUuggAUGAAGCAUACguGGuCCGg- -3' miRNA: 3'- caACGA---UACUUCGUAUGguCU-GGUac -5' |
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32780 | 5' | -47.6 | NC_007346.1 | + | 279657 | 0.68 | 0.999928 |
Target: 5'- cUUGCUGUGAauAGUAUGCCccACCGa- -3' miRNA: 3'- cAACGAUACU--UCGUAUGGucUGGUac -5' |
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32780 | 5' | -47.6 | NC_007346.1 | + | 248546 | 0.7 | 0.9996 |
Target: 5'- -aUGUUAcucGAGUAUGCCGGACCAg- -3' miRNA: 3'- caACGAUac-UUCGUAUGGUCUGGUac -5' |
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32780 | 5' | -47.6 | NC_007346.1 | + | 190820 | 0.73 | 0.995582 |
Target: 5'- --aGCcgGUGucacAGCAUGCCAGACCGg- -3' miRNA: 3'- caaCGa-UACu---UCGUAUGGUCUGGUac -5' |
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32780 | 5' | -47.6 | NC_007346.1 | + | 261133 | 0.8 | 0.851765 |
Target: 5'- -aUGCUGcUGAAGCAUACCGGuCCGUc -3' miRNA: 3'- caACGAU-ACUUCGUAUGGUCuGGUAc -5' |
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32780 | 5' | -47.6 | NC_007346.1 | + | 217324 | 1.1 | 0.033213 |
Target: 5'- cGUUGCUAUGAAGCAUACCAGACCAUGa -3' miRNA: 3'- -CAACGAUACUUCGUAUGGUCUGGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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