Results 1 - 19 of 19 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32781 | 5' | -51.1 | NC_007346.1 | + | 160222 | 0.66 | 0.999924 |
Target: 5'- aCCGGCAgCGCCGCcgg-UAUCGgaUAUCg -3' miRNA: 3'- -GGCUGU-GCGGCGaaagAUAGCg-AUGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 311194 | 0.66 | 0.999903 |
Target: 5'- aCCGcCAuuacCGCCGCcaUCUccAUCGCcauUACCg -3' miRNA: 3'- -GGCuGU----GCGGCGaaAGA--UAGCG---AUGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 98004 | 0.66 | 0.999876 |
Target: 5'- uCCGugcgcuCACGCUGCaaUUUCUGUgGUU-CCg -3' miRNA: 3'- -GGCu-----GUGCGGCG--AAAGAUAgCGAuGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 257159 | 0.66 | 0.999876 |
Target: 5'- gCCGuguCACGCUGCUcgUCaa--GCUGCUu -3' miRNA: 3'- -GGCu--GUGCGGCGAa-AGauagCGAUGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 257756 | 0.66 | 0.99984 |
Target: 5'- gCGGCACGCCGgaaa--GUCGCUgagugggACCg -3' miRNA: 3'- gGCUGUGCGGCgaaagaUAGCGA-------UGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 287501 | 0.67 | 0.999753 |
Target: 5'- uCCGGCauaaACGCCGUUgcaUCguuUCGaaCUACCa -3' miRNA: 3'- -GGCUG----UGCGGCGAa--AGau-AGC--GAUGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 275139 | 0.67 | 0.999693 |
Target: 5'- --aACACGCCGCaca----CGCUGCCa -3' miRNA: 3'- ggcUGUGCGGCGaaagauaGCGAUGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 239147 | 0.67 | 0.999686 |
Target: 5'- gCUGACgAUGCCGaa-UCUGUCGauugacaCUACCa -3' miRNA: 3'- -GGCUG-UGCGGCgaaAGAUAGC-------GAUGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 386215 | 0.68 | 0.999429 |
Target: 5'- cCCGGCaugccaaaacaGCGCCGCUgccccUCGUUACa -3' miRNA: 3'- -GGCUG-----------UGCGGCGAaagauAGCGAUGg -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 135247 | 0.68 | 0.999305 |
Target: 5'- aCUGGCuucuuUGCCGCcUUCU-UCGCcGCCu -3' miRNA: 3'- -GGCUGu----GCGGCGaAAGAuAGCGaUGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 244468 | 0.68 | 0.999159 |
Target: 5'- aCGcACGCGCuCGCUUUCUugcaaagcaCGC-ACCa -3' miRNA: 3'- gGC-UGUGCG-GCGAAAGAua-------GCGaUGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 371202 | 0.68 | 0.998987 |
Target: 5'- cCCGugcCugGCCGagaaUUUGUCGcCUACCa -3' miRNA: 3'- -GGCu--GugCGGCgaa-AGAUAGC-GAUGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 257883 | 0.7 | 0.996729 |
Target: 5'- aCgGACACGCCGCguagaUUCccgCGCaucUGCCc -3' miRNA: 3'- -GgCUGUGCGGCGa----AAGauaGCG---AUGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 253296 | 0.7 | 0.996198 |
Target: 5'- uUGGCACGCCGgUUUUugUAUCGacACCg -3' miRNA: 3'- gGCUGUGCGGCgAAAG--AUAGCgaUGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 78029 | 0.7 | 0.995599 |
Target: 5'- cCCGGCGCGgCGCUUUgaGUUG--GCCa -3' miRNA: 3'- -GGCUGUGCgGCGAAAgaUAGCgaUGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 349377 | 0.7 | 0.994925 |
Target: 5'- gCCGcCAcCGCCGCcgUC-GUCGCcACCa -3' miRNA: 3'- -GGCuGU-GCGGCGaaAGaUAGCGaUGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 264100 | 0.74 | 0.963368 |
Target: 5'- aCCGAgACGCCGU---CUAUCGCgucucauuguucccaUACCg -3' miRNA: 3'- -GGCUgUGCGGCGaaaGAUAGCG---------------AUGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 260709 | 0.75 | 0.938504 |
Target: 5'- gCG-CGCGCCGCUUUUUGcgGCUAUCg -3' miRNA: 3'- gGCuGUGCGGCGAAAGAUagCGAUGG- -5' |
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32781 | 5' | -51.1 | NC_007346.1 | + | 238664 | 1.16 | 0.010082 |
Target: 5'- aCCGACACGCCGCUUUCUAUCGCUACCa -3' miRNA: 3'- -GGCUGUGCGGCGAAAGAUAGCGAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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