Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32782 | 5' | -55.4 | NC_007346.1 | + | 274308 | 0.66 | 0.992523 |
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Target: 5'- cGAGCgcggCGUCUUgGUCGUCGCgC-UGa -3' miRNA: 3'- aCUUGa---GCGGAGgUAGCAGCGgGuAC- -5' |
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| 32782 | 5' | -55.4 | NC_007346.1 | + | 349382 | 0.66 | 0.987821 |
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Target: 5'- --cAC-CGCCgCCGUCGUCGCCa--- -3' miRNA: 3'- acuUGaGCGGaGGUAGCAGCGGguac -5' |
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| 32782 | 5' | -55.4 | NC_007346.1 | + | 293033 | 0.67 | 0.979057 |
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Target: 5'- cGAACgcguauaCGCCUCCAgUCGUgaaCGCUUAUGc -3' miRNA: 3'- aCUUGa------GCGGAGGU-AGCA---GCGGGUAC- -5' |
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| 32782 | 5' | -55.4 | NC_007346.1 | + | 57944 | 0.68 | 0.963217 |
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Target: 5'- -----cCGCCUCCAUCGcCGCCUc-- -3' miRNA: 3'- acuugaGCGGAGGUAGCaGCGGGuac -5' |
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| 32782 | 5' | -55.4 | NC_007346.1 | + | 364359 | 0.7 | 0.935972 |
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Target: 5'- aUGGuACUCGCaaCUCCcUCGUuacagCGCCCAUGc -3' miRNA: 3'- -ACU-UGAGCG--GAGGuAGCA-----GCGGGUAC- -5' |
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| 32782 | 5' | -55.4 | NC_007346.1 | + | 238912 | 1.08 | 0.010761 |
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Target: 5'- aUGAACUCGCCUCCAUCGUCGCCCAUGc -3' miRNA: 3'- -ACUUGAGCGGAGGUAGCAGCGGGUAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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