Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32783 | 3' | -48.4 | NC_007346.1 | + | 111348 | 0.66 | 0.999994 |
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Target: 5'- aAUGCCccgGGAUCaUGUUGAUuUGACACa- -3' miRNA: 3'- cUACGG---CUUAG-ACAGCUA-ACUGUGau -5' |
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| 32783 | 3' | -48.4 | NC_007346.1 | + | 258879 | 0.68 | 0.999885 |
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Target: 5'- uGGUGgCGAGUUguggaGUUGGUUGACGCg- -3' miRNA: 3'- -CUACgGCUUAGa----CAGCUAACUGUGau -5' |
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| 32783 | 3' | -48.4 | NC_007346.1 | + | 243455 | 0.69 | 0.999536 |
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Target: 5'- uGGUGgCGAGUUgucgaGUUGAUUGACGCg- -3' miRNA: 3'- -CUACgGCUUAGa----CAGCUAACUGUGau -5' |
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| 32783 | 3' | -48.4 | NC_007346.1 | + | 5918 | 0.72 | 0.996476 |
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Target: 5'- -uUGCCGcacGUUUGUUGGUUGAUGCUGu -3' miRNA: 3'- cuACGGCu--UAGACAGCUAACUGUGAU- -5' |
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| 32783 | 3' | -48.4 | NC_007346.1 | + | 388513 | 0.72 | 0.995214 |
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Target: 5'- --aGCUGGAUUUGcCGAUUGACAUg- -3' miRNA: 3'- cuaCGGCUUAGACaGCUAACUGUGau -5' |
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| 32783 | 3' | -48.4 | NC_007346.1 | + | 239142 | 1.08 | 0.043265 |
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Target: 5'- cGAUGCCGAAUCUGUCGAUUGACACUAc -3' miRNA: 3'- -CUACGGCUUAGACAGCUAACUGUGAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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