miRNA display CGI


Results 1 - 14 of 14 are showing below:
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ID Location Perfect MFE.* RefSeq Acc. strand Start Position R_P_ratio# P value
Predicted miRNA align pattern
32785 5' -46.2 NC_007346.1 + 260312 0.66 1
Target:  5'- gCGCGGAGGucGGGUacccgUUGUCGuggCUCa -3'
miRNA:   3'- -GCGCCUCU--UCUAa----AGCAGUuuaGAGc -5'
32785 5' -46.2 NC_007346.1 + 188162 0.67 1
Target:  5'- --gGGGGAAGugaaGUUUCGUCAccgauuGGUCUUGg -3'
miRNA:   3'- gcgCCUCUUC----UAAAGCAGU------UUAGAGC- -5'
32785 5' -46.2 NC_007346.1 + 280128 0.66 1
Target:  5'- -uUGGAGAAGGUucguucUUUGUCGGAUCg-- -3'
miRNA:   3'- gcGCCUCUUCUA------AAGCAGUUUAGagc -5'
32785 5' -46.2 NC_007346.1 + 306223 0.66 1
Target:  5'- gGUGGAGGAGAUggCgGUgGAGUCgcCGg -3'
miRNA:   3'- gCGCCUCUUCUAaaG-CAgUUUAGa-GC- -5'
32785 5' -46.2 NC_007346.1 + 317138 0.68 0.999999
Target:  5'- -cCGGAGAAGAa---GUCGcAUCUCGu -3'
miRNA:   3'- gcGCCUCUUCUaaagCAGUuUAGAGC- -5'
32785 5' -46.2 NC_007346.1 + 274485 0.71 0.999949
Target:  5'- gGCGGucauGAcGAUUUCGUCAAG-CUUGc -3'
miRNA:   3'- gCGCCu---CUuCUAAAGCAGUUUaGAGC- -5'
32785 5' -46.2 NC_007346.1 + 223846 0.73 0.99932
Target:  5'- aGCGGGGGacgcauugaaGGcgUUCG-CGAAUCUCGu -3'
miRNA:   3'- gCGCCUCU----------UCuaAAGCaGUUUAGAGC- -5'
32785 5' -46.2 NC_007346.1 + 303310 0.74 0.998783
Target:  5'- aCGCGGAGggGAUUgUGUCAc-UUUCa -3'
miRNA:   3'- -GCGCCUCuuCUAAaGCAGUuuAGAGc -5'
32785 5' -46.2 NC_007346.1 + 259086 0.76 0.995169
Target:  5'- aCGCGGAGGaauauuccuuuuauGGGUUUCGUUaauGAGUUUCGu -3'
miRNA:   3'- -GCGCCUCU--------------UCUAAAGCAG---UUUAGAGC- -5'
32785 5' -46.2 NC_007346.1 + 258316 0.76 0.995169
Target:  5'- aCGCGGAGGaauauuccuuccauGGGUUUCGUUAAuggGUUUCGu -3'
miRNA:   3'- -GCGCCUCU--------------UCUAAAGCAGUU---UAGAGC- -5'
32785 5' -46.2 NC_007346.1 + 256971 0.76 0.995169
Target:  5'- aCGCGGAGGaauauuccuucuauGGGUUUCGUUAAuggGUUUCGu -3'
miRNA:   3'- -GCGCCUCU--------------UCUAAAGCAGUU---UAGAGC- -5'
32785 5' -46.2 NC_007346.1 + 257709 0.76 0.993826
Target:  5'- aCGCGGAGGaauauuccuucuGGGUUUCGUUAAuggGUUUCGu -3'
miRNA:   3'- -GCGCCUCU------------UCUAAAGCAGUU---UAGAGC- -5'
32785 5' -46.2 NC_007346.1 + 269586 0.95 0.350903
Target:  5'- uGCGGAGAAGGUUaCGUCAAAUCUCa -3'
miRNA:   3'- gCGCCUCUUCUAAaGCAGUUUAGAGc -5'
32785 5' -46.2 NC_007346.1 + 256049 1.12 0.052671
Target:  5'- uCGCGGAGAAGAUUUCGUCAAAUCUCGg -3'
miRNA:   3'- -GCGCCUCUUCUAAAGCAGUUUAGAGC- -5' 
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Note:
      When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.

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TDL, Institute of Biomedical Science, Academia Sinica, Taipei, Taiwan.
Copyright © 2007 TDL. All rights reserved.