Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32786 | 3' | -54.2 | NC_007346.1 | + | 305893 | 0.66 | 0.998417 |
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Target: 5'- gGCGAGACgGaaGggggugGugGAGACgGUGa -3' miRNA: 3'- -UGCUCUGgCagCaa----CugCUCUGgCAC- -5' |
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| 32786 | 3' | -54.2 | NC_007346.1 | + | 98689 | 0.66 | 0.997003 |
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Target: 5'- uGCGAGcCCGUUcUUGACGuGGCCc-- -3' miRNA: 3'- -UGCUCuGGCAGcAACUGCuCUGGcac -5' |
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| 32786 | 3' | -54.2 | NC_007346.1 | + | 257752 | 0.67 | 0.995974 |
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Target: 5'- cACGccGGAaaGUCGcUGAgUGGGACCGUGa -3' miRNA: 3'- -UGC--UCUggCAGCaACU-GCUCUGGCAC- -5' |
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| 32786 | 3' | -54.2 | NC_007346.1 | + | 109449 | 0.68 | 0.991056 |
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Target: 5'- aACGAuGAUCGUCGUgGACGGGGUCGc- -3' miRNA: 3'- -UGCU-CUGGCAGCAaCUGCUCUGGCac -5' |
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| 32786 | 3' | -54.2 | NC_007346.1 | + | 109397 | 0.7 | 0.965315 |
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Target: 5'- cCGGGAaccCCGUCGUcgUGACGAuGAUCGUc -3' miRNA: 3'- uGCUCU---GGCAGCA--ACUGCU-CUGGCAc -5' |
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| 32786 | 3' | -54.2 | NC_007346.1 | + | 269515 | 0.74 | 0.85698 |
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Target: 5'- gACGAGAUUGaCGagaUUGACGAGACCGa- -3' miRNA: 3'- -UGCUCUGGCaGC---AACUGCUCUGGCac -5' |
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| 32786 | 3' | -54.2 | NC_007346.1 | + | 256107 | 0.8 | 0.589317 |
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Target: 5'- gACGAGACCGaCGaaaccaugaUUGACGAGACCGa- -3' miRNA: 3'- -UGCUCUGGCaGC---------AACUGCUCUGGCac -5' |
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| 32786 | 3' | -54.2 | NC_007346.1 | + | 256251 | 0.8 | 0.551499 |
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Target: 5'- gACGAGAcacagcCCG-CGaUUGACGAGACCGUGa -3' miRNA: 3'- -UGCUCU------GGCaGC-AACUGCUCUGGCAC- -5' |
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| 32786 | 3' | -54.2 | NC_007346.1 | + | 256164 | 0.97 | 0.077756 |
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Target: 5'- gACGAGAcacagcCCGUCGUUGACGAGACCGUGa -3' miRNA: 3'- -UGCUCU------GGCAGCAACUGCUCUGGCAC- -5' |
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| 32786 | 3' | -54.2 | NC_007346.1 | + | 256194 | 1.09 | 0.014392 |
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Target: 5'- gACGAGACCGUCGUUGACGAGACCGUGa -3' miRNA: 3'- -UGCUCUGGCAGCAACUGCUCUGGCAC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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