Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32786 | 5' | -55.4 | NC_007346.1 | + | 326192 | 0.66 | 0.993463 |
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Target: 5'- cGACgACGGCaagagCGUCGUUGAUuuaaugcucgaaGAGAUCGa -3' miRNA: 3'- -CUG-UGUCGg----GCAGCAACUG------------CUCUGGC- -5' |
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| 32786 | 5' | -55.4 | NC_007346.1 | + | 87076 | 0.68 | 0.974824 |
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Target: 5'- uGCGCAGCgaCGUCGUUGuCGAgcGACgCGa -3' miRNA: 3'- cUGUGUCGg-GCAGCAACuGCU--CUG-GC- -5' |
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| 32786 | 5' | -55.4 | NC_007346.1 | + | 261023 | 0.71 | 0.922571 |
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Target: 5'- cGACACggaAGCCCGUCuuUUGGC-AGACCa -3' miRNA: 3'- -CUGUG---UCGGGCAGc-AACUGcUCUGGc -5' |
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| 32786 | 5' | -55.4 | NC_007346.1 | + | 98696 | 0.71 | 0.911852 |
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Target: 5'- cGGCGCcugcgAGCCCGUUcUUGACGuGGCCc -3' miRNA: 3'- -CUGUG-----UCGGGCAGcAACUGCuCUGGc -5' |
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| 32786 | 5' | -55.4 | NC_007346.1 | + | 86366 | 0.73 | 0.830977 |
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Target: 5'- cGCGCGGUgaCGUUGUUgGACGAGACCu -3' miRNA: 3'- cUGUGUCGg-GCAGCAA-CUGCUCUGGc -5' |
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| 32786 | 5' | -55.4 | NC_007346.1 | + | 256247 | 0.92 | 0.104191 |
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Target: 5'- aGACACAGCCCG-CGaUUGACGAGACCGu -3' miRNA: 3'- -CUGUGUCGGGCaGC-AACUGCUCUGGC- -5' |
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| 32786 | 5' | -55.4 | NC_007346.1 | + | 256124 | 0.93 | 0.092093 |
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Target: 5'- aGACACAGCCCG-CGaUUGACGAGACCGa -3' miRNA: 3'- -CUGUGUCGGGCaGC-AACUGCUCUGGC- -5' |
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| 32786 | 5' | -55.4 | NC_007346.1 | + | 256160 | 1.08 | 0.011099 |
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Target: 5'- aGACACAGCCCGUCGUUGACGAGACCGu -3' miRNA: 3'- -CUGUGUCGGGCAGCAACUGCUCUGGC- -5' |
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| 32786 | 5' | -55.4 | NC_007346.1 | + | 256211 | 1.08 | 0.011099 |
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Target: 5'- aGACACAGCCCGUCGUUGACGAGACCGu -3' miRNA: 3'- -CUGUGUCGGGCAGCAACUGCUCUGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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