Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32788 | 5' | -49.8 | NC_007346.1 | + | 393229 | 0.67 | 0.999876 |
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Target: 5'- cGuCAGGAUggUCAGCCAaagcgcccAGUUUCcgGGUUCa -3' miRNA: 3'- uC-GUCUUA--AGUCGGU--------UCAGAG--CCAAG- -5' |
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| 32788 | 5' | -49.8 | NC_007346.1 | + | 86732 | 0.71 | 0.993492 |
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Target: 5'- cGGCGGAGggugCGGCCAauGGUCUCaGUa- -3' miRNA: 3'- -UCGUCUUaa--GUCGGU--UCAGAGcCAag -5' |
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| 32788 | 5' | -49.8 | NC_007346.1 | + | 259439 | 0.79 | 0.811341 |
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Target: 5'- uAGCAGAAUUCAGCCcauuGG--UCGGUUCc -3' miRNA: 3'- -UCGUCUUAAGUCGGu---UCagAGCCAAG- -5' |
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| 32788 | 5' | -49.8 | NC_007346.1 | + | 257324 | 1.09 | 0.024738 |
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Target: 5'- uAGCAGAAUUCAGCCAAGUCUCGGUUCc -3' miRNA: 3'- -UCGUCUUAAGUCGGUUCAGAGCCAAG- -5' |
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| 32788 | 5' | -49.8 | NC_007346.1 | + | 258062 | 1.09 | 0.024738 |
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Target: 5'- uAGCAGAAUUCAGCCAAGUCUCGGUUCc -3' miRNA: 3'- -UCGUCUUAAGUCGGUUCAGAGCCAAG- -5' |
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| 32788 | 5' | -49.8 | NC_007346.1 | + | 258668 | 1.09 | 0.024738 |
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Target: 5'- uAGCAGAAUUCAGCCAAGUCUCGGUUCc -3' miRNA: 3'- -UCGUCUUAAGUCGGUUCAGAGCCAAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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