Results 1 - 5 of 5 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32789 | 3' | -56.3 | NC_007346.1 | + | 257859 | 1.1 | 0.007463 |
Target: 5'- aCAAGGCACCAUCCGGACGGUCCAUCGg -3' miRNA: 3'- -GUUCCGUGGUAGGCCUGCCAGGUAGC- -5' |
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32789 | 3' | -56.3 | NC_007346.1 | + | 98281 | 0.69 | 0.945125 |
Target: 5'- -cAGGCGCCG-CCGG-CGGUUCAa-- -3' miRNA: 3'- guUCCGUGGUaGGCCuGCCAGGUagc -5' |
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32789 | 3' | -56.3 | NC_007346.1 | + | 101824 | 0.66 | 0.986179 |
Target: 5'- gCAAGuGCGuuGUUCGGACGGaUCCGg-- -3' miRNA: 3'- -GUUC-CGUggUAGGCCUGCC-AGGUagc -5' |
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32789 | 3' | -56.3 | NC_007346.1 | + | 51379 | 0.66 | 0.986179 |
Target: 5'- aCGAaGCACCAca-GGACGGUCgCAUUGc -3' miRNA: 3'- -GUUcCGUGGUaggCCUGCCAG-GUAGC- -5' |
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32789 | 3' | -56.3 | NC_007346.1 | + | 162821 | 0.66 | 0.987648 |
Target: 5'- aAAGuGCACCAUCaccaaguaGAUGGUUCAUUGa -3' miRNA: 3'- gUUC-CGUGGUAGgc------CUGCCAGGUAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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