Results 1 - 4 of 4 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32795 | 3' | -56.4 | NC_007346.1 | + | 371132 | 0.66 | 0.990165 |
Target: 5'- -cGGGUGCAacuccgucggcUGUGCAUGGaaagUCgGCUGCUg -3' miRNA: 3'- gcCCCAUGU-----------ACGCGUGCC----AG-CGAUGG- -5' |
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32795 | 3' | -56.4 | NC_007346.1 | + | 284106 | 0.67 | 0.971864 |
Target: 5'- uGGGuGUACAUGUGCAUcuUCGagUACCu -3' miRNA: 3'- gCCC-CAUGUACGCGUGccAGCg-AUGG- -5' |
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32795 | 3' | -56.4 | NC_007346.1 | + | 134104 | 0.69 | 0.945236 |
Target: 5'- aGGGGauUGCAacCGCACGG-CGC-ACCg -3' miRNA: 3'- gCCCC--AUGUacGCGUGCCaGCGaUGG- -5' |
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32795 | 3' | -56.4 | NC_007346.1 | + | 263658 | 1.12 | 0.005073 |
Target: 5'- gCGGGGUACAUGCGCACGGUCGCUACCa -3' miRNA: 3'- -GCCCCAUGUACGCGUGCCAGCGAUGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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