miRNA: 3'- -UUACGUCUGUCACgCGCGguuaACCG- -5'
miRNA: 3'- uUACGUCUGUCACGC-GCgGuuaaCCG- -5'
miRNA: 3'- -UUACguCUGUCACg--CGCgGUUa-----ACCG- -5'
miRNA: 3'- uuaCguCUG-UCACGCGcGGUUa--ACCG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32796 | 3' | -53.1 | NC_007346.1 | + | 201146 | 0.66 | 0.997784 |
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Target: 5'- cGGUGCAGAUGGUG-GUGCaga-UGGUa -3' miRNA: 3'- -UUACGUCUGUCACgCGCGguuaACCG- -5' |
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| 32796 | 3' | -53.1 | NC_007346.1 | + | 266665 | 0.66 | 0.997784 |
|
Target: 5'- cAUGUGGACAGUGCGgUGgCuc--GGCa -3' miRNA: 3'- uUACGUCUGUCACGC-GCgGuuaaCCG- -5' |
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| 32796 | 3' | -53.1 | NC_007346.1 | + | 189452 | 0.66 | 0.997784 |
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Target: 5'- uGGUGguGACGGUGgugGCGgCGAcgguggUGGCg -3' miRNA: 3'- -UUACguCUGUCACg--CGCgGUUa-----ACCG- -5' |
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| 32796 | 3' | -53.1 | NC_007346.1 | + | 248636 | 0.66 | 0.997784 |
|
Target: 5'- --aGguGACuGGUGCGU-CCGAgagUGGCu -3' miRNA: 3'- uuaCguCUG-UCACGCGcGGUUa--ACCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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