Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32797 | 3' | -52.9 | NC_007346.1 | + | 312106 | 0.69 | 0.989629 |
Target: 5'- gUGCUCACaacaucUUCUCAUGCC-CGUCa -3' miRNA: 3'- gGCGGGUGgu----AAGAGUAUGGuGCAGc -5' |
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32797 | 3' | -52.9 | NC_007346.1 | + | 302217 | 0.7 | 0.981783 |
Target: 5'- -gGCCCACCAcugUCUCcuuggcauAUGCCACGg-- -3' miRNA: 3'- ggCGGGUGGUa--AGAG--------UAUGGUGCagc -5' |
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32797 | 3' | -52.9 | NC_007346.1 | + | 305586 | 0.7 | 0.979786 |
Target: 5'- aCC-CCCACCAUcgacuuccccaUCUCcaccGCCACGUCc -3' miRNA: 3'- -GGcGGGUGGUA-----------AGAGua--UGGUGCAGc -5' |
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32797 | 3' | -52.9 | NC_007346.1 | + | 392930 | 0.73 | 0.922216 |
Target: 5'- gCGCCCACCAggaaaagUCAagaagacUACCACGUCu -3' miRNA: 3'- gGCGGGUGGUaag----AGU-------AUGGUGCAGc -5' |
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32797 | 3' | -52.9 | NC_007346.1 | + | 255408 | 0.79 | 0.687325 |
Target: 5'- gCGCCCGCCAUUgcUAUACCACGUg- -3' miRNA: 3'- gGCGGGUGGUAAgaGUAUGGUGCAgc -5' |
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32797 | 3' | -52.9 | NC_007346.1 | + | 270228 | 1.1 | 0.014616 |
Target: 5'- cCCGCCCACCAUUCUCAUACCACGUCGc -3' miRNA: 3'- -GGCGGGUGGUAAGAGUAUGGUGCAGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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