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ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position![]() |
R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
3280 | 5' | -43.2 | NC_001543.1 | + | 1187 | 1.09 | 0.001534 |
Target: 5'- gCCCAACAACAGUGUAAUCACAACUUUc -3' miRNA: 3'- -GGGUUGUUGUCACAUUAGUGUUGAAA- -5' |
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3280 | 5' | -43.2 | NC_001543.1 | + | 1189 | 0.69 | 0.650361 |
Target: 5'- aCCCGACAACAGUGgaAAagACAAa--- -3' miRNA: 3'- -GGGUUGUUGUCACa-UUagUGUUgaaa -5' |
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3280 | 5' | -43.2 | NC_001543.1 | + | 3711 | 0.72 | 0.45872 |
Target: 5'- cCCCAACAACuugccAGUGUGuAUgGCAACa-- -3' miRNA: 3'- -GGGUUGUUG-----UCACAU-UAgUGUUGaaa -5' |
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3280 | 5' | -43.2 | NC_001543.1 | + | 6672 | 0.67 | 0.784967 |
Target: 5'- aCCCAcagguGCAGCggcaggagugccgGGUGUGGUUACAAUUc- -3' miRNA: 3'- -GGGU-----UGUUG-------------UCACAUUAGUGUUGAaa -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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