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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32800 | 3' | -51.2 | NC_007346.1 | + | 361550 | 0.66 | 0.999928 |
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Target: 5'- --cGAGGAGGcgugUGAUaaCUCCGAUacCGGAu -3' miRNA: 3'- gaaUUCCUCCu---GCUA--GAGGCUA--GCCU- -5' |
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| 32800 | 3' | -51.2 | NC_007346.1 | + | 259941 | 0.66 | 0.9999 |
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Target: 5'- gUUGAGGAGauggcuaGCGAUCUCgCGcgcgauauccaaucGUCGGAg -3' miRNA: 3'- gAAUUCCUCc------UGCUAGAG-GC--------------UAGCCU- -5' |
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| 32800 | 3' | -51.2 | NC_007346.1 | + | 390255 | 0.66 | 0.999849 |
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Target: 5'- --gGAGGAGGACGAguauaUgUCCGAUg--- -3' miRNA: 3'- gaaUUCCUCCUGCU-----AgAGGCUAgccu -5' |
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| 32800 | 3' | -51.2 | NC_007346.1 | + | 376290 | 0.66 | 0.999809 |
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Target: 5'- -------uGGACGAUCUC-GAUCGGGu -3' miRNA: 3'- gaauuccuCCUGCUAGAGgCUAGCCU- -5' |
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| 32800 | 3' | -51.2 | NC_007346.1 | + | 274642 | 0.66 | 0.999761 |
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Target: 5'- ---nAGGuuGAgGAUCUCCGAUCcGAg -3' miRNA: 3'- gaauUCCucCUgCUAGAGGCUAGcCU- -5' |
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| 32800 | 3' | -51.2 | NC_007346.1 | + | 183858 | 0.66 | 0.999761 |
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Target: 5'- uUUGGGGAGGAUGGUaaUCCaGAUgGGu -3' miRNA: 3'- gAAUUCCUCCUGCUAg-AGG-CUAgCCu -5' |
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| 32800 | 3' | -51.2 | NC_007346.1 | + | 342790 | 0.69 | 0.997235 |
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Target: 5'- aCUUAcu--GGACGAUCaUCCGAUgGGAu -3' miRNA: 3'- -GAAUuccuCCUGCUAG-AGGCUAgCCU- -5' |
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| 32800 | 3' | -51.2 | NC_007346.1 | + | 274667 | 1.1 | 0.021226 |
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Target: 5'- cCUUAAGGAGGACGAUCUCCGAUCGGAg -3' miRNA: 3'- -GAAUUCCUCCUGCUAGAGGCUAGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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