Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32802 | 3' | -47.2 | NC_007346.1 | + | 86557 | 0.7 | 0.999843 |
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Target: 5'- gGAGcGCGAcuugauAAUGCaCGAGUUGAGcUGCg -3' miRNA: 3'- -CUC-CGCU------UUACG-GUUCAAUUCuACGg -5' |
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| 32802 | 3' | -47.2 | NC_007346.1 | + | 250440 | 0.7 | 0.999799 |
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Target: 5'- -uGGCGAGuUGUgGAGUUGAcuGAUGCg -3' miRNA: 3'- cuCCGCUUuACGgUUCAAUU--CUACGg -5' |
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| 32802 | 3' | -47.2 | NC_007346.1 | + | 32091 | 0.7 | 0.999681 |
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Target: 5'- -uGGCGGA--GCCGucuuAGUgAGGGUGCCg -3' miRNA: 3'- cuCCGCUUuaCGGU----UCAaUUCUACGG- -5' |
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| 32802 | 3' | -47.2 | NC_007346.1 | + | 275319 | 0.7 | 0.999681 |
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Target: 5'- uGGGUGAGAcgccGCCGGGUuugggUGAGAcGCCg -3' miRNA: 3'- cUCCGCUUUa---CGGUUCA-----AUUCUaCGG- -5' |
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| 32802 | 3' | -47.2 | NC_007346.1 | + | 275277 | 0.7 | 0.999681 |
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Target: 5'- uGGGUGAGAcgccGCCGGGUuugggUGAGAcGCCg -3' miRNA: 3'- cUCCGCUUUa---CGGUUCA-----AUUCUaCGG- -5' |
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| 32802 | 3' | -47.2 | NC_007346.1 | + | 262626 | 0.72 | 0.999084 |
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Target: 5'- -uGGCGAGuUGCgGAGUUGgcuGAUGCg -3' miRNA: 3'- cuCCGCUUuACGgUUCAAUu--CUACGg -5' |
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| 32802 | 3' | -47.2 | NC_007346.1 | + | 256768 | 0.72 | 0.999084 |
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Target: 5'- -uGGCGAGuUGCgGAGUUGgcuGAUGCg -3' miRNA: 3'- cuCCGCUUuACGgUUCAAUu--CUACGg -5' |
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| 32802 | 3' | -47.2 | NC_007346.1 | + | 255455 | 0.73 | 0.99681 |
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Target: 5'- -uGGCGAAAagcugGCCGgcAGUUGGGAuacccUGCCg -3' miRNA: 3'- cuCCGCUUUa----CGGU--UCAAUUCU-----ACGG- -5' |
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| 32802 | 3' | -47.2 | NC_007346.1 | + | 275119 | 1.14 | 0.02386 |
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Target: 5'- uGAGGCGAAAUGCCAAGUUAAGAUGCCa -3' miRNA: 3'- -CUCCGCUUUACGGUUCAAUUCUACGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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