Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32804 | 5' | -51.5 | NC_007346.1 | + | 308915 | 0.66 | 0.999631 |
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Target: 5'- cGGUggaGUAGGCGGAGGCGgagaUggCggUGGa -3' miRNA: 3'- -UCAg--CAUCCGCUUCCGCg---AaaGa-ACC- -5' |
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| 32804 | 5' | -51.5 | NC_007346.1 | + | 364810 | 0.66 | 0.999545 |
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Target: 5'- aAGcCGUGGGCauGGGCGCUguaaCgaGGg -3' miRNA: 3'- -UCaGCAUCCGcuUCCGCGAaa--GaaCC- -5' |
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| 32804 | 5' | -51.5 | NC_007346.1 | + | 310725 | 0.68 | 0.998296 |
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Target: 5'- uGGUCGggguGGCGcAGGCGgUgugggugggCUUGGg -3' miRNA: 3'- -UCAGCau--CCGCuUCCGCgAaa-------GAACC- -5' |
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| 32804 | 5' | -51.5 | NC_007346.1 | + | 338388 | 0.68 | 0.997982 |
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Target: 5'- -uUCGUGGGUGGGcGCGCgcgUCUgGGa -3' miRNA: 3'- ucAGCAUCCGCUUcCGCGaa-AGAaCC- -5' |
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| 32804 | 5' | -51.5 | NC_007346.1 | + | 171880 | 0.68 | 0.997621 |
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Target: 5'- gGGUaCGUGGGCGAcuGCGacgucuCUUUCUUGu -3' miRNA: 3'- -UCA-GCAUCCGCUucCGC------GAAAGAACc -5' |
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| 32804 | 5' | -51.5 | NC_007346.1 | + | 124046 | 0.7 | 0.988834 |
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Target: 5'- uGUUGUAGGUGAAGGUGgaU--UUGGu -3' miRNA: 3'- uCAGCAUCCGCUUCCGCgaAagAACC- -5' |
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| 32804 | 5' | -51.5 | NC_007346.1 | + | 58741 | 0.72 | 0.979075 |
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Target: 5'- cAGuUUGUAGGCGGuggagcugcaauuggAGGUGCUUUUggUGGg -3' miRNA: 3'- -UC-AGCAUCCGCU---------------UCCGCGAAAGa-ACC- -5' |
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| 32804 | 5' | -51.5 | NC_007346.1 | + | 170191 | 0.72 | 0.978192 |
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Target: 5'- -uUCGgcGGCgGAGGGCGCUUUUUUc- -3' miRNA: 3'- ucAGCauCCG-CUUCCGCGAAAGAAcc -5' |
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| 32804 | 5' | -51.5 | NC_007346.1 | + | 309623 | 0.77 | 0.83511 |
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Target: 5'- uGcCGUAGGaGAAuGCGCUUUCUUGGg -3' miRNA: 3'- uCaGCAUCCgCUUcCGCGAAAGAACC- -5' |
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| 32804 | 5' | -51.5 | NC_007346.1 | + | 307053 | 1.11 | 0.017095 |
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Target: 5'- uAGUCGUAGGCGAAGGCGCUUUCUUGGu -3' miRNA: 3'- -UCAGCAUCCGCUUCCGCGAAAGAACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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