Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32805 | 3' | -59.7 | NC_007346.1 | + | 259900 | 0.66 | 0.933691 |
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Target: 5'- cCGCGCGAGUucuuuuuUGUCCgACGGuGGCCAUu- -3' miRNA: 3'- -GCGUGCUCA-------GCGGG-UGCU-CCGGUGug -5' |
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| 32805 | 3' | -59.7 | NC_007346.1 | + | 234507 | 0.67 | 0.914315 |
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Target: 5'- gGCugaguCGcGUCGCCC-CccGGCCGCGCg -3' miRNA: 3'- gCGu----GCuCAGCGGGuGcuCCGGUGUG- -5' |
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| 32805 | 3' | -59.7 | NC_007346.1 | + | 137664 | 0.67 | 0.891313 |
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Target: 5'- cCGCcCGAGUgaaCGCCCAgggaacCGAuauGCCACACg -3' miRNA: 3'- -GCGuGCUCA---GCGGGU------GCUc--CGGUGUG- -5' |
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| 32805 | 3' | -59.7 | NC_007346.1 | + | 87185 | 0.68 | 0.878008 |
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Target: 5'- cCGCGCGGGcCGCcgucuuacugagaCCAUu-GGCCGCACc -3' miRNA: 3'- -GCGUGCUCaGCG-------------GGUGcuCCGGUGUG- -5' |
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| 32805 | 3' | -59.7 | NC_007346.1 | + | 275292 | 0.68 | 0.851184 |
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Target: 5'- uGCACGGGgaCGCCCAgcauuCGAaccaaagcGGUCACGCg -3' miRNA: 3'- gCGUGCUCa-GCGGGU-----GCU--------CCGGUGUG- -5' |
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| 32805 | 3' | -59.7 | NC_007346.1 | + | 373033 | 0.69 | 0.843887 |
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Target: 5'- uGCGCGAGUCGUaUCGCu-GGUCACAUu -3' miRNA: 3'- gCGUGCUCAGCG-GGUGcuCCGGUGUG- -5' |
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| 32805 | 3' | -59.7 | NC_007346.1 | + | 39979 | 0.71 | 0.736604 |
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Target: 5'- uGC-CGGGUCgaGCCCAgCGGGuGCCACAg -3' miRNA: 3'- gCGuGCUCAG--CGGGU-GCUC-CGGUGUg -5' |
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| 32805 | 3' | -59.7 | NC_007346.1 | + | 47979 | 0.74 | 0.579199 |
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Target: 5'- uGUugGAGUCGUCCAauucauGGCCAUGCa -3' miRNA: 3'- gCGugCUCAGCGGGUgcu---CCGGUGUG- -5' |
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| 32805 | 3' | -59.7 | NC_007346.1 | + | 307086 | 1.07 | 0.005741 |
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Target: 5'- gCGCACGAGUCGCCCACGAGGCCACAUa -3' miRNA: 3'- -GCGUGCUCAGCGGGUGCUCCGGUGUG- -5' |
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| 32805 | 3' | -59.7 | NC_007346.1 | + | 309651 | 1.1 | 0.003635 |
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Target: 5'- gCGCACGAGUCGCCCACGAGGCCACACa -3' miRNA: 3'- -GCGUGCUCAGCGGGUGCUCCGGUGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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