Results 1 - 6 of 6 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32806 | 5' | -47.9 | NC_007346.1 | + | 87701 | 0.67 | 0.999967 |
Target: 5'- gAAGCacucgcuuuUCGCGCUCGA-GUCgAUCAAc- -3' miRNA: 3'- aUUCG---------AGCGCGAGUUaCAG-UAGUUau -5' |
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32806 | 5' | -47.9 | NC_007346.1 | + | 135284 | 0.68 | 0.999869 |
Target: 5'- -uGGCUCGCGCUCA--GUCuUCuuUGu -3' miRNA: 3'- auUCGAGCGCGAGUuaCAGuAGuuAU- -5' |
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32806 | 5' | -47.9 | NC_007346.1 | + | 278104 | 0.69 | 0.999729 |
Target: 5'- cAAGCgUUGCGUcgaUCGAUGUCGUUAAc- -3' miRNA: 3'- aUUCG-AGCGCG---AGUUACAGUAGUUau -5' |
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32806 | 5' | -47.9 | NC_007346.1 | + | 135829 | 0.69 | 0.999729 |
Target: 5'- cGGGCUCGgGCUCuGUGguggCGUCGu-- -3' miRNA: 3'- aUUCGAGCgCGAGuUACa---GUAGUuau -5' |
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32806 | 5' | -47.9 | NC_007346.1 | + | 243534 | 0.7 | 0.998807 |
Target: 5'- --uGCacgCGCGCUCAAUGUCggCGGa- -3' miRNA: 3'- auuCGa--GCGCGAGUUACAGuaGUUau -5' |
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32806 | 5' | -47.9 | NC_007346.1 | + | 323498 | 1.06 | 0.052359 |
Target: 5'- uUAAGCUCGCGCUCAAUGUCAUCAAUAu -3' miRNA: 3'- -AUUCGAGCGCGAGUUACAGUAGUUAU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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