Results 1 - 10 of 10 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32808 | 3' | -52.6 | NC_007346.1 | + | 219913 | 0.66 | 0.998953 |
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Target: 5'- -uGGAucuGGAUCUGGAUCUuuCGUGGAc-- -3' miRNA: 3'- guCCU---UCUAGGCCUAGG--GUACCUugu -5' |
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| 32808 | 3' | -52.6 | NC_007346.1 | + | 271655 | 0.66 | 0.998894 |
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Target: 5'- uCAGGucaGUCCGGGUCCCGuauccuugugcauuUGGAu-- -3' miRNA: 3'- -GUCCuucUAGGCCUAGGGU--------------ACCUugu -5' |
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| 32808 | 3' | -52.6 | NC_007346.1 | + | 62883 | 0.67 | 0.997863 |
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Target: 5'- aAGGAGguGAUCCGGAcauuucuUCCguCAUGGaAGCAa -3' miRNA: 3'- gUCCUU--CUAGGCCU-------AGG--GUACC-UUGU- -5' |
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| 32808 | 3' | -52.6 | NC_007346.1 | + | 123990 | 0.68 | 0.995448 |
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Target: 5'- gUAGGAGGAUCCGGcauaggggcagGUUCCGguacgGGGAg- -3' miRNA: 3'- -GUCCUUCUAGGCC-----------UAGGGUa----CCUUgu -5' |
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| 32808 | 3' | -52.6 | NC_007346.1 | + | 182468 | 0.68 | 0.995448 |
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Target: 5'- cCAGGAGGAgCCGGAUCgg--GGAAUc -3' miRNA: 3'- -GUCCUUCUaGGCCUAGgguaCCUUGu -5' |
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| 32808 | 3' | -52.6 | NC_007346.1 | + | 351020 | 0.68 | 0.994751 |
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Target: 5'- gGGGAAGAuguuUCUGGAggagCCgAUGGuGCAu -3' miRNA: 3'- gUCCUUCU----AGGCCUa---GGgUACCuUGU- -5' |
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| 32808 | 3' | -52.6 | NC_007346.1 | + | 399303 | 0.69 | 0.991053 |
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Target: 5'- -cGGAAGAcuuUCCGGAUCCCGccuaaAACAa -3' miRNA: 3'- guCCUUCU---AGGCCUAGGGUacc--UUGU- -5' |
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| 32808 | 3' | -52.6 | NC_007346.1 | + | 62053 | 0.69 | 0.985554 |
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Target: 5'- uCAGGAAG-UCUGG--UCCAUGGAAUg -3' miRNA: 3'- -GUCCUUCuAGGCCuaGGGUACCUUGu -5' |
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| 32808 | 3' | -52.6 | NC_007346.1 | + | 327614 | 0.7 | 0.983842 |
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Target: 5'- aGGGGAGAUCUGGAaaguaUUCCAUcgaGGAugGu -3' miRNA: 3'- gUCCUUCUAGGCCU-----AGGGUA---CCUugU- -5' |
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| 32808 | 3' | -52.6 | NC_007346.1 | + | 336816 | 1.09 | 0.016019 |
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Target: 5'- aCAGGAAGAUCCGGAUCCCAUGGAACAu -3' miRNA: 3'- -GUCCUUCUAGGCCUAGGGUACCUUGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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