miRNA: 3'- -UCAACGA--------CUGCGUGGG----UGACAGCUGc -5'
miRNA: 3'- -UCAACGACUg--CGUGGGUG----ACAGCUGc -5'
miRNA: 3'- uCAACGac--------UGCGUGGGUgACAGCUGC- -5'
miRNA: 3'- ucaacGACugCGUGGGUGAC---AGCUGc -5'
miRNA: 3'- -UCAACGACUGCGUGGGUGACAGCUGC- -5'
Results 1 - 5 of 5 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32811 | 5' | -54.7 | NC_007346.1 | + | 242291 | 0.66 | 0.995884 |
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Target: 5'- cAGUUGCUuuuugccaGACGCGUCCauguACUGUUGAUa -3' miRNA: 3'- -UCAACGA--------CUGCGUGGG----UGACAGCUGc -5' |
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| 32811 | 5' | -54.7 | NC_007346.1 | + | 401566 | 0.68 | 0.979786 |
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Target: 5'- uGGUUGUUGAgcgGCGCCCGCggcuUGUUGAUu -3' miRNA: 3'- -UCAACGACUg--CGUGGGUG----ACAGCUGc -5' |
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| 32811 | 5' | -54.7 | NC_007346.1 | + | 243545 | 0.7 | 0.953974 |
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Target: 5'- cGUUGCgucauugcacGCGCGCUCAaUGUCGGCGg -3' miRNA: 3'- uCAACGac--------UGCGUGGGUgACAGCUGC- -5' |
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| 32811 | 5' | -54.7 | NC_007346.1 | + | 39543 | 0.72 | 0.90021 |
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Target: 5'- ----cCUGugGCACCCGCUGggcUCGACc -3' miRNA: 3'- ucaacGACugCGUGGGUGAC---AGCUGc -5' |
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| 32811 | 5' | -54.7 | NC_007346.1 | + | 392634 | 1.08 | 0.012171 |
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Target: 5'- aAGUUGCUGACGCACCCACUGUCGACGc -3' miRNA: 3'- -UCAACGACUGCGUGGGUGACAGCUGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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