Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32814 | 3' | -46 | NC_007346.1 | + | 171908 | 0.66 | 1 |
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Target: 5'- cUGGUGcgUCUGGUUcucCGAACaCGGCGGg -3' miRNA: 3'- -AUCGUaaAGACUAAa--GCUUG-GUCGUC- -5' |
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| 32814 | 3' | -46 | NC_007346.1 | + | 272805 | 0.66 | 1 |
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Target: 5'- -uGCAUUcaUCUucacgUCGAACCAGUGGa -3' miRNA: 3'- auCGUAA--AGAcuaa-AGCUUGGUCGUC- -5' |
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| 32814 | 3' | -46 | NC_007346.1 | + | 196981 | 0.67 | 1 |
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Target: 5'- -uGCGuUUUCUGGaaUUUCGAcgaaACCAGguGa -3' miRNA: 3'- auCGU-AAAGACU--AAAGCU----UGGUCguC- -5' |
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| 32814 | 3' | -46 | NC_007346.1 | + | 303824 | 0.68 | 0.999998 |
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Target: 5'- gUAGUAUUUUUGAUUuuUUGGGCCAcuGUAGc -3' miRNA: 3'- -AUCGUAAAGACUAA--AGCUUGGU--CGUC- -5' |
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| 32814 | 3' | -46 | NC_007346.1 | + | 381073 | 0.68 | 0.999998 |
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Target: 5'- aAGCAUUUCUGAUUgcccaGAAaaaAGCAa -3' miRNA: 3'- aUCGUAAAGACUAAag---CUUgg-UCGUc -5' |
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| 32814 | 3' | -46 | NC_007346.1 | + | 195881 | 0.68 | 0.999997 |
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Target: 5'- aAGCAUugauccaucuaUUCuUGGUUUUGAACCAGUc- -3' miRNA: 3'- aUCGUA-----------AAG-ACUAAAGCUUGGUCGuc -5' |
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| 32814 | 3' | -46 | NC_007346.1 | + | 39546 | 0.69 | 0.999993 |
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Target: 5'- gUGGCAcccgCUGGgcUCGAcCCGGCAGc -3' miRNA: 3'- -AUCGUaaa-GACUaaAGCUuGGUCGUC- -5' |
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| 32814 | 3' | -46 | NC_007346.1 | + | 43405 | 1.09 | 0.055419 |
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Target: 5'- aUAGCAUUUCUGAUUUCGAACCAGCAGa -3' miRNA: 3'- -AUCGUAAAGACUAAAGCUUGGUCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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