Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32814 | 5' | -50.9 | NC_007346.1 | + | 274378 | 0.66 | 0.999901 |
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Target: 5'- ----uUCGGUGCAUGGAAUC--CUGGa -3' miRNA: 3'- cugacAGCCACGUGCUUUGGuuGACC- -5' |
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| 32814 | 5' | -50.9 | NC_007346.1 | + | 247747 | 0.66 | 0.999873 |
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Target: 5'- aGAUaGUaGGUGCAaaauAACCGGCUGGu -3' miRNA: 3'- -CUGaCAgCCACGUgcu-UUGGUUGACC- -5' |
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| 32814 | 5' | -50.9 | NC_007346.1 | + | 86373 | 0.67 | 0.999516 |
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Target: 5'- uGAC-GUUGuUGgACGAGACCuGCUGGa -3' miRNA: 3'- -CUGaCAGCcACgUGCUUUGGuUGACC- -5' |
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| 32814 | 5' | -50.9 | NC_007346.1 | + | 109488 | 0.67 | 0.999408 |
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Target: 5'- uGCUGgcgUGGUgGCAU---ACCAACUGGg -3' miRNA: 3'- cUGACa--GCCA-CGUGcuuUGGUUGACC- -5' |
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| 32814 | 5' | -50.9 | NC_007346.1 | + | 138821 | 0.7 | 0.993009 |
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Target: 5'- cACUGcauUCGGUGCGuuucACCGACUGGa -3' miRNA: 3'- cUGAC---AGCCACGUgcuuUGGUUGACC- -5' |
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| 32814 | 5' | -50.9 | NC_007346.1 | + | 38365 | 0.71 | 0.990932 |
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Target: 5'- -cCUGuUUGGUGgGuCGAAAUCAACUGGu -3' miRNA: 3'- cuGAC-AGCCACgU-GCUUUGGUUGACC- -5' |
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| 32814 | 5' | -50.9 | NC_007346.1 | + | 39582 | 0.93 | 0.201206 |
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Target: 5'- uGGCUGUCGGUGCACuAGACCAACUGa -3' miRNA: 3'- -CUGACAGCCACGUGcUUUGGUUGACc -5' |
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| 32814 | 5' | -50.9 | NC_007346.1 | + | 43440 | 1.12 | 0.016814 |
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Target: 5'- uGACUGUCGGUGCACGAAACCAACUGGg -3' miRNA: 3'- -CUGACAGCCACGUGCUUUGGUUGACC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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