Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32821 | 3' | -53.7 | NC_007346.1 | + | 85305 | 0.66 | 0.996499 |
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Target: 5'- gAACUCCU--GGUGUCCAUUGAcguacuuUCCAa -3' miRNA: 3'- -UUGAGGGcgCCGUAGGUAACUu------AGGU- -5' |
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| 32821 | 3' | -53.7 | NC_007346.1 | + | 6240 | 0.66 | 0.995931 |
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Target: 5'- uAACUCCCG-GGUgaaCCAUUGAuuggauuuacAUCCAc -3' miRNA: 3'- -UUGAGGGCgCCGua-GGUAACU----------UAGGU- -5' |
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| 32821 | 3' | -53.7 | NC_007346.1 | + | 60031 | 0.67 | 0.991859 |
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Target: 5'- cAGCUCCC-CGGguUCCcaUGAAUCa- -3' miRNA: 3'- -UUGAGGGcGCCguAGGuaACUUAGgu -5' |
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| 32821 | 3' | -53.7 | NC_007346.1 | + | 134168 | 0.67 | 0.990749 |
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Target: 5'- ---gUCCGCGGCGcgUCCAUcUGAaacAUCCAg -3' miRNA: 3'- uugaGGGCGCCGU--AGGUA-ACU---UAGGU- -5' |
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| 32821 | 3' | -53.7 | NC_007346.1 | + | 296191 | 0.68 | 0.985075 |
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Target: 5'- cAGCcCUCGUGGCGUCCAUuucuuuuuuUG-AUCCAu -3' miRNA: 3'- -UUGaGGGCGCCGUAGGUA---------ACuUAGGU- -5' |
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| 32821 | 3' | -53.7 | NC_007346.1 | + | 145428 | 0.69 | 0.969259 |
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Target: 5'- cGACUCCagGUGcCAUCCGUUGAAUCa- -3' miRNA: 3'- -UUGAGGg-CGCcGUAGGUAACUUAGgu -5' |
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| 32821 | 3' | -53.7 | NC_007346.1 | + | 227498 | 0.79 | 0.565309 |
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Target: 5'- uAGCUUCCGCGGCcacagcaGUCCAUUGggUCg- -3' miRNA: 3'- -UUGAGGGCGCCG-------UAGGUAACuuAGgu -5' |
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| 32821 | 3' | -53.7 | NC_007346.1 | + | 157233 | 1.06 | 0.016676 |
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Target: 5'- uAACUCCCGCGGCAUCCAUUGAAUCCAu -3' miRNA: 3'- -UUGAGGGCGCCGUAGGUAACUUAGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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