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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32821 | 5' | -56.7 | NC_007346.1 | + | 266933 | 0.66 | 0.982648 |
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Target: 5'- -gUGGCGAGuUGUGGAuuugcuaucUGCaCUCG-GGAc -3' miRNA: 3'- gaACCGUUC-ACGCCU---------ACG-GAGCgCCU- -5' |
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| 32821 | 5' | -56.7 | NC_007346.1 | + | 267224 | 0.66 | 0.982648 |
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Target: 5'- -gUGGCGAGuUGUGGAuuugcuaucUGCaCUCG-GGAc -3' miRNA: 3'- gaACCGUUC-ACGCCU---------ACG-GAGCgCCU- -5' |
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| 32821 | 5' | -56.7 | NC_007346.1 | + | 309213 | 0.66 | 0.980734 |
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Target: 5'- --cGGCAuGUGUG---GCCUCGUGGGc -3' miRNA: 3'- gaaCCGUuCACGCcuaCGGAGCGCCU- -5' |
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| 32821 | 5' | -56.7 | NC_007346.1 | + | 256767 | 0.67 | 0.962055 |
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Target: 5'- -gUGGCGAGuUGCGGAguugGCUgaugcgaccgagCGUGGGu -3' miRNA: 3'- gaACCGUUC-ACGCCUa---CGGa-----------GCGCCU- -5' |
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| 32821 | 5' | -56.7 | NC_007346.1 | + | 392183 | 0.68 | 0.952195 |
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Target: 5'- uUUGGCuuuGGUGgGGGUGCgUCgacaGUGGGg -3' miRNA: 3'- gAACCGu--UCACgCCUACGgAG----CGCCU- -5' |
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| 32821 | 5' | -56.7 | NC_007346.1 | + | 289875 | 0.7 | 0.88517 |
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Target: 5'- uCUUGGUAAGUGgGGAUcacauguugacGUCUCuGCGGc -3' miRNA: 3'- -GAACCGUUCACgCCUA-----------CGGAG-CGCCu -5' |
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| 32821 | 5' | -56.7 | NC_007346.1 | + | 242587 | 0.76 | 0.598732 |
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Target: 5'- ---uGCcauGGUGCGGAUGCuCUCGUGGAg -3' miRNA: 3'- gaacCGu--UCACGCCUACG-GAGCGCCU- -5' |
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| 32821 | 5' | -56.7 | NC_007346.1 | + | 157269 | 1.09 | 0.006495 |
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Target: 5'- cCUUGGCAAGUGCGGAUGCCUCGCGGAg -3' miRNA: 3'- -GAACCGUUCACGCCUACGGAGCGCCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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