Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32822 | 5' | -53.8 | NC_007346.1 | + | 258377 | 0.66 | 0.997365 |
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Target: 5'- gGCGgAUcUCGGCGA--UCCucGGCCc -3' miRNA: 3'- -UGCgUAcAGCCGCUuuAGGuuCCGGu -5' |
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| 32822 | 5' | -53.8 | NC_007346.1 | + | 275126 | 0.66 | 0.997323 |
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Target: 5'- gACGC-UGUgaGGCGAAAUgCCAAGuuaagauGCCAu -3' miRNA: 3'- -UGCGuACAg-CCGCUUUA-GGUUC-------CGGU- -5' |
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| 32822 | 5' | -53.8 | NC_007346.1 | + | 253095 | 0.66 | 0.995715 |
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Target: 5'- cAC-CAUGUCGGCGugaaguuGGAUCgCAaucggggAGGCCAu -3' miRNA: 3'- -UGcGUACAGCCGC-------UUUAG-GU-------UCCGGU- -5' |
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| 32822 | 5' | -53.8 | NC_007346.1 | + | 274543 | 0.67 | 0.994465 |
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Target: 5'- gGCGCAauuUCGGCGAu-UCgGAGGCg- -3' miRNA: 3'- -UGCGUac-AGCCGCUuuAGgUUCCGgu -5' |
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| 32822 | 5' | -53.8 | NC_007346.1 | + | 216575 | 0.67 | 0.992748 |
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Target: 5'- cGCGCGaaUGUCGGCGu--UUCGccGCCGg -3' miRNA: 3'- -UGCGU--ACAGCCGCuuuAGGUucCGGU- -5' |
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| 32822 | 5' | -53.8 | NC_007346.1 | + | 86631 | 0.67 | 0.992652 |
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Target: 5'- gGCGCGUGUcacacagaagcCGGCGGucugguggcgugcGGUCCAGGcGCUu -3' miRNA: 3'- -UGCGUACA-----------GCCGCU-------------UUAGGUUC-CGGu -5' |
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| 32822 | 5' | -53.8 | NC_007346.1 | + | 12092 | 0.69 | 0.976995 |
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Target: 5'- aAUGCccggGUCGGgGAAAUCUAuaauucuuGGCCAg -3' miRNA: 3'- -UGCGua--CAGCCgCUUUAGGUu-------CCGGU- -5' |
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| 32822 | 5' | -53.8 | NC_007346.1 | + | 171241 | 1.09 | 0.012046 |
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Target: 5'- gACGCAUGUCGGCGAAAUCCAAGGCCAa -3' miRNA: 3'- -UGCGUACAGCCGCUUUAGGUUCCGGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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