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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32824 | 3' | -48.9 | NC_007346.1 | + | 182805 | 0.66 | 0.999975 |
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Target: 5'- aCCAucUCCA---UCUGCAUGUGCAGc- -3' miRNA: 3'- -GGUc-AGGUcuaAGACGUAUACGUCuu -5' |
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| 32824 | 3' | -48.9 | NC_007346.1 | + | 347947 | 0.67 | 0.999901 |
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Target: 5'- gCGGUCUccacuGGAUUCaUGUAgGUGCAGAu -3' miRNA: 3'- gGUCAGG-----UCUAAG-ACGUaUACGUCUu -5' |
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| 32824 | 3' | -48.9 | NC_007346.1 | + | 303257 | 0.68 | 0.999671 |
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Target: 5'- aCCAGguaCAGAUUCauacacagggGCAUGUGCAcGAGa -3' miRNA: 3'- -GGUCag-GUCUAAGa---------CGUAUACGU-CUU- -5' |
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| 32824 | 3' | -48.9 | NC_007346.1 | + | 397091 | 0.73 | 0.98692 |
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Target: 5'- aCCGGUCCAGGaUCUGCAU-UGUAu-- -3' miRNA: 3'- -GGUCAGGUCUaAGACGUAuACGUcuu -5' |
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| 32824 | 3' | -48.9 | NC_007346.1 | + | 209956 | 0.8 | 0.813157 |
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Target: 5'- uCCGGaagaCCAuugguGAUUCUGCAUAUGCAGAAu -3' miRNA: 3'- -GGUCa---GGU-----CUAAGACGUAUACGUCUU- -5' |
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| 32824 | 3' | -48.9 | NC_007346.1 | + | 209505 | 1.08 | 0.032133 |
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Target: 5'- uCCAGUCCAGAUUCUGCAUAUGCAGAAu -3' miRNA: 3'- -GGUCAGGUCUAAGACGUAUACGUCUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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