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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32824 | 5' | -51.8 | NC_007346.1 | + | 389215 | 0.67 | 0.998827 |
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Target: 5'- --aUgCGGAuaauacaUGUGAUGCCGCAUUgAUa -3' miRNA: 3'- agaAgGCCU-------ACACUAUGGCGUAGgUA- -5' |
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| 32824 | 5' | -51.8 | NC_007346.1 | + | 316205 | 0.67 | 0.99862 |
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Target: 5'- cUUUCCGGA-GUGAaACCGUcacuugaugauAUCCAg -3' miRNA: 3'- aGAAGGCCUaCACUaUGGCG-----------UAGGUa -5' |
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| 32824 | 5' | -51.8 | NC_007346.1 | + | 363132 | 0.67 | 0.998047 |
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Target: 5'- ---cCCGGAUGuUGAUGCCGCGa---- -3' miRNA: 3'- agaaGGCCUAC-ACUAUGGCGUaggua -5' |
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| 32824 | 5' | -51.8 | NC_007346.1 | + | 287555 | 0.68 | 0.99677 |
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Target: 5'- gCUUCUGGuAUGUGuuuUGCCGCGgaugcauaugacaUCCAc -3' miRNA: 3'- aGAAGGCC-UACACu--AUGGCGU-------------AGGUa -5' |
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| 32824 | 5' | -51.8 | NC_007346.1 | + | 280182 | 0.69 | 0.991407 |
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Target: 5'- --aUCCGGAUGauAUGCauuCGCAUCCAUg -3' miRNA: 3'- agaAGGCCUACacUAUG---GCGUAGGUA- -5' |
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| 32824 | 5' | -51.8 | NC_007346.1 | + | 209541 | 1.07 | 0.022526 |
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Target: 5'- gUCUUCCGGAUGUGAUACCGCAUCCAUa -3' miRNA: 3'- -AGAAGGCCUACACUAUGGCGUAGGUA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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