Results 1 - 9 of 9 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32825 | 5' | -53.3 | NC_007346.1 | + | 304929 | 0.66 | 0.997845 |
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Target: 5'- -cUCCCGGUGgguACACaUGCCAacaAUACg-- -3' miRNA: 3'- acAGGGCCAC---UGUG-ACGGU---UAUGaca -5' |
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| 32825 | 5' | -53.3 | NC_007346.1 | + | 96118 | 0.67 | 0.992952 |
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Target: 5'- -aUCUCGGUGACAUuuuuUGUCucUACUGUc -3' miRNA: 3'- acAGGGCCACUGUG----ACGGuuAUGACA- -5' |
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| 32825 | 5' | -53.3 | NC_007346.1 | + | 160145 | 0.68 | 0.989666 |
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Target: 5'- cGU-CCGGUGccacguccuGCAUUGCCcAAUACUGg -3' miRNA: 3'- aCAgGGCCAC---------UGUGACGG-UUAUGACa -5' |
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| 32825 | 5' | -53.3 | NC_007346.1 | + | 236907 | 0.7 | 0.960335 |
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Target: 5'- -uUCCCGGUGACAuCUG-CGAUGCa-- -3' miRNA: 3'- acAGGGCCACUGU-GACgGUUAUGaca -5' |
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| 32825 | 5' | -53.3 | NC_007346.1 | + | 160646 | 0.71 | 0.956802 |
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Target: 5'- ---aCCGGcGGCGCUGCCGGUACa-- -3' miRNA: 3'- acagGGCCaCUGUGACGGUUAUGaca -5' |
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| 32825 | 5' | -53.3 | NC_007346.1 | + | 131966 | 0.71 | 0.944936 |
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Target: 5'- aGUCUCaGUGGCACacuUGCCAAUACg-- -3' miRNA: 3'- aCAGGGcCACUGUG---ACGGUUAUGaca -5' |
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| 32825 | 5' | -53.3 | NC_007346.1 | + | 201494 | 0.72 | 0.935942 |
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Target: 5'- aUGUUgCGGuUGAUGCUGUUGAUGCUGUu -3' miRNA: 3'- -ACAGgGCC-ACUGUGACGGUUAUGACA- -5' |
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| 32825 | 5' | -53.3 | NC_007346.1 | + | 215493 | 0.74 | 0.848676 |
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Target: 5'- aGUCCCuGUGAauuauUACUGCUggUGCUGg -3' miRNA: 3'- aCAGGGcCACU-----GUGACGGuuAUGACa -5' |
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| 32825 | 5' | -53.3 | NC_007346.1 | + | 211491 | 1.09 | 0.013908 |
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Target: 5'- aUGUCCCGGUGACACUGCCAAUACUGUg -3' miRNA: 3'- -ACAGGGCCACUGUGACGGUUAUGACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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