miRNA: 3'- cUUUGAG-CUGUuuCCaACAUGUGCGg -5'
miRNA: 3'- cuuuGAGCUGUu--UCCa---ACaUGUGCGG- -5'
miRNA: 3'- -CUUUGAGCUGUUuCCAACaug--UGCgG- -5'
miRNA: 3'- -CUUUGAGCUGUUUCCAACAUGUGCGG- -5'
Results 21 - 24 of 24 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32826 | 3' | -46.2 | NC_007346.1 | + | 284119 | 0.7 | 0.999969 |
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Target: 5'- cAAACUCgGACAuuGGgUGUACAUGUg -3' miRNA: 3'- cUUUGAG-CUGUuuCCaACAUGUGCGg -5' |
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| 32826 | 3' | -46.2 | NC_007346.1 | + | 91368 | 0.7 | 0.999959 |
|
Target: 5'- ---uUUCGACAuguGGGgaagUG-ACACGCCa -3' miRNA: 3'- cuuuGAGCUGUu--UCCa---ACaUGUGCGG- -5' |
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| 32826 | 3' | -46.2 | NC_007346.1 | + | 249003 | 0.73 | 0.999521 |
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Target: 5'- aGAAACUCGACGAuGGUUGcgguaACGgCg -3' miRNA: 3'- -CUUUGAGCUGUUuCCAACaug--UGCgG- -5' |
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| 32826 | 3' | -46.2 | NC_007346.1 | + | 230692 | 1.15 | 0.033562 |
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Target: 5'- gGAAACUCGACAAAGGUUGUACACGCCg -3' miRNA: 3'- -CUUUGAGCUGUUUCCAACAUGUGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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