Results 1 - 6 of 6 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32828 | 3' | -54.9 | NC_007346.1 | + | 202517 | 0.67 | 0.98501 |
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Target: 5'- gUgGuGGAgUCGAUUACggaUGUGACCGAc -3' miRNA: 3'- aAgCuCCUgAGCUAGUG---GCACUGGCU- -5' |
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| 32828 | 3' | -54.9 | NC_007346.1 | + | 264360 | 0.71 | 0.911222 |
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Target: 5'- -gCGcaaGGGACaugCGGUCACCGaGACCGAc -3' miRNA: 3'- aaGC---UCCUGa--GCUAGUGGCaCUGGCU- -5' |
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| 32828 | 3' | -54.9 | NC_007346.1 | + | 61001 | 0.74 | 0.787867 |
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Target: 5'- gUUGAGGAUUUGGUCACCGacGCUGAc -3' miRNA: 3'- aAGCUCCUGAGCUAGUGGCacUGGCU- -5' |
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| 32828 | 3' | -54.9 | NC_007346.1 | + | 239355 | 0.76 | 0.705166 |
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Target: 5'- cUCGAuggugauGGugUCGGUCACgGUGAUCGAg -3' miRNA: 3'- aAGCU-------CCugAGCUAGUGgCACUGGCU- -5' |
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| 32828 | 3' | -54.9 | NC_007346.1 | + | 190272 | 0.77 | 0.667994 |
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Target: 5'- -aCGAGGACaUCGAUUACCGUGAgUGu -3' miRNA: 3'- aaGCUCCUG-AGCUAGUGGCACUgGCu -5' |
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| 32828 | 3' | -54.9 | NC_007346.1 | + | 238906 | 1.06 | 0.015628 |
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Target: 5'- gUUCGAGGACUCGAUCACCGUGACCGAc -3' miRNA: 3'- -AAGCUCCUGAGCUAGUGGCACUGGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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