Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32832 | 5' | -50.9 | NC_007346.1 | + | 46975 | 0.66 | 0.99965 |
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Target: 5'- cUUGCGCGAggcGUCgCCAUUGUGauUGUu -3' miRNA: 3'- -AACGCGCUau-UAG-GGUAACGCguACA- -5' |
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| 32832 | 5' | -50.9 | NC_007346.1 | + | 370784 | 0.66 | 0.999565 |
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Target: 5'- -cGCGUGAccGUCCUucuGUUggcGCGCGUGUa -3' miRNA: 3'- aaCGCGCUauUAGGG---UAA---CGCGUACA- -5' |
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| 32832 | 5' | -50.9 | NC_007346.1 | + | 208276 | 0.66 | 0.999565 |
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Target: 5'- aUGCGUGGUAA-CCCAaUUGUGCu--- -3' miRNA: 3'- aACGCGCUAUUaGGGU-AACGCGuaca -5' |
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| 32832 | 5' | -50.9 | NC_007346.1 | + | 260726 | 0.67 | 0.998816 |
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Target: 5'- gUGCGCGGUG--UCCAUUaGCGCGc-- -3' miRNA: 3'- aACGCGCUAUuaGGGUAA-CGCGUaca -5' |
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| 32832 | 5' | -50.9 | NC_007346.1 | + | 263947 | 0.69 | 0.994767 |
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Target: 5'- cUUGCGCGuuaagaauucgGUAAUCUCAaUGCGCuuuGUGUg -3' miRNA: 3'- -AACGCGC-----------UAUUAGGGUaACGCG---UACA- -5' |
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| 32832 | 5' | -50.9 | NC_007346.1 | + | 166123 | 0.69 | 0.993056 |
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Target: 5'- --cUGUGAUAAUCCgGUacaauaaguUGCGCAUGUg -3' miRNA: 3'- aacGCGCUAUUAGGgUA---------ACGCGUACA- -5' |
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| 32832 | 5' | -50.9 | NC_007346.1 | + | 38505 | 0.7 | 0.985143 |
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Target: 5'- -cGUGCGAUAuccgcAUCCCAUuuuUGCGUAUa- -3' miRNA: 3'- aaCGCGCUAU-----UAGGGUA---ACGCGUAca -5' |
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| 32832 | 5' | -50.9 | NC_007346.1 | + | 255284 | 1.05 | 0.032587 |
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Target: 5'- uUUGCGCGAUAAUCCCAUUGCGCAUGUc -3' miRNA: 3'- -AACGCGCUAUUAGGGUAACGCGUACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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