Results 1 - 10 of 10 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
32833 | 3' | -50.5 | NC_007346.1 | + | 27123 | 0.66 | 0.999896 |
Target: 5'- aUGCaAAucUGGACGCCuugcaauuuuuGCGCGCGUUGu -3' miRNA: 3'- cGCGaUU--ACCUGUGG-----------CGCGUGUAAUu -5' |
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32833 | 3' | -50.5 | NC_007346.1 | + | 318197 | 0.67 | 0.999579 |
Target: 5'- gGUGCagUAAUGGAUGCUGCacaGCGCGUg-- -3' miRNA: 3'- -CGCG--AUUACCUGUGGCG---CGUGUAauu -5' |
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32833 | 3' | -50.5 | NC_007346.1 | + | 238407 | 0.67 | 0.99948 |
Target: 5'- aGCGCUugccaccgaggAGUacauuaacGGGCGCCGCGCA-GUUAAg -3' miRNA: 3'- -CGCGA-----------UUA--------CCUGUGGCGCGUgUAAUU- -5' |
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32833 | 3' | -50.5 | NC_007346.1 | + | 87764 | 0.68 | 0.998044 |
Target: 5'- gGgGUcuAUGGACGCCGCGCuuACAc--- -3' miRNA: 3'- -CgCGauUACCUGUGGCGCG--UGUaauu -5' |
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32833 | 3' | -50.5 | NC_007346.1 | + | 86460 | 0.69 | 0.996253 |
Target: 5'- uGCGCUAAcccuccaGGAUgacuuugguGCCGCGCACGa--- -3' miRNA: 3'- -CGCGAUUa------CCUG---------UGGCGCGUGUaauu -5' |
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32833 | 3' | -50.5 | NC_007346.1 | + | 134606 | 0.69 | 0.996253 |
Target: 5'- -aGCUggauguuucaGAUGGACgcGCCGCGgACAUUGu -3' miRNA: 3'- cgCGA----------UUACCUG--UGGCGCgUGUAAUu -5' |
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32833 | 3' | -50.5 | NC_007346.1 | + | 252273 | 0.7 | 0.994166 |
Target: 5'- uGCGCUGAUaaaucgcgaGGAgACCGCGCGa----- -3' miRNA: 3'- -CGCGAUUA---------CCUgUGGCGCGUguaauu -5' |
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32833 | 3' | -50.5 | NC_007346.1 | + | 315224 | 0.7 | 0.993292 |
Target: 5'- gGCGCUGAuuauauUGGAguaCGCCGCGaaACGUUAAa -3' miRNA: 3'- -CGCGAUU------ACCU---GUGGCGCg-UGUAAUU- -5' |
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32833 | 3' | -50.5 | NC_007346.1 | + | 110105 | 0.71 | 0.985633 |
Target: 5'- gGCuGCUGAUGGugAugUCGCGUACGUa-- -3' miRNA: 3'- -CG-CGAUUACCugU--GGCGCGUGUAauu -5' |
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32833 | 3' | -50.5 | NC_007346.1 | + | 260299 | 1.09 | 0.020729 |
Target: 5'- cGCGCUAAUGGACACCGCGCACAUUAAg -3' miRNA: 3'- -CGCGAUUACCUGUGGCGCGUGUAAUU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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