Results 1 - 8 of 8 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32834 | 3' | -52.9 | NC_007346.1 | + | 402975 | 0.66 | 0.997839 |
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Target: 5'- aUAGCUCCgGaaugGUcacUGCCAUuggACCGGCAc -3' miRNA: 3'- -GUCGAGGaCga--CA---AUGGUA---UGGUCGU- -5' |
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| 32834 | 3' | -52.9 | NC_007346.1 | + | 150345 | 0.66 | 0.997009 |
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Target: 5'- --aCUCCUGUgauu-CUAUACCAGCAg -3' miRNA: 3'- gucGAGGACGacaauGGUAUGGUCGU- -5' |
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| 32834 | 3' | -52.9 | NC_007346.1 | + | 306556 | 0.67 | 0.993738 |
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Target: 5'- cCAGCUCCUGCcuccucgaUGacucuuccgUACCGaACCAGUg -3' miRNA: 3'- -GUCGAGGACG--------ACa--------AUGGUaUGGUCGu -5' |
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| 32834 | 3' | -52.9 | NC_007346.1 | + | 182887 | 0.68 | 0.988066 |
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Target: 5'- cCGGCUCCUcCUGgauUACCAagcacacuuCCAGCAa -3' miRNA: 3'- -GUCGAGGAcGACa--AUGGUau-------GGUCGU- -5' |
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| 32834 | 3' | -52.9 | NC_007346.1 | + | 389589 | 0.68 | 0.986402 |
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Target: 5'- -uGcCUCCUGUUGUUACUGUACCuuuauuuGGUAg -3' miRNA: 3'- guC-GAGGACGACAAUGGUAUGG-------UCGU- -5' |
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| 32834 | 3' | -52.9 | NC_007346.1 | + | 401677 | 0.68 | 0.984913 |
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Target: 5'- aAGUUCCUGCUGUaGCCuaGCC-GUAu -3' miRNA: 3'- gUCGAGGACGACAaUGGuaUGGuCGU- -5' |
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| 32834 | 3' | -52.9 | NC_007346.1 | + | 312341 | 0.92 | 0.130716 |
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Target: 5'- -uGCaCCUGCUGUUACCAUACCAGCAg -3' miRNA: 3'- guCGaGGACGACAAUGGUAUGGUCGU- -5' |
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| 32834 | 3' | -52.9 | NC_007346.1 | + | 304475 | 1.09 | 0.011726 |
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Target: 5'- aCAGCUCCUGCUGUUACCAUACCAGCAg -3' miRNA: 3'- -GUCGAGGACGACAAUGGUAUGGUCGU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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