Results 1 - 7 of 7 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 32835 | 3' | -54.7 | NC_007346.1 | + | 170062 | 0.66 | 0.996018 |
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Target: 5'- gCAUCCGGAaauCCAUcucgGGAu--GGCGGa -3' miRNA: 3'- gGUAGGCCU---GGUAca--CCUuucCCGCC- -5' |
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| 32835 | 3' | -54.7 | NC_007346.1 | + | 188036 | 0.7 | 0.965827 |
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Target: 5'- ----gUGGGCgGcgGUGGGGAGGGCGGc -3' miRNA: 3'- gguagGCCUGgUa-CACCUUUCCCGCC- -5' |
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| 32835 | 3' | -54.7 | NC_007346.1 | + | 184858 | 0.72 | 0.915709 |
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Target: 5'- uCCAUCCgccugGGAUgAgg-GGAAGGGGUGGg -3' miRNA: 3'- -GGUAGG-----CCUGgUacaCCUUUCCCGCC- -5' |
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| 32835 | 3' | -54.7 | NC_007346.1 | + | 138171 | 0.73 | 0.892448 |
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Target: 5'- gCGUCCGGACCGg--GGcgcacGAGcGGCGGa -3' miRNA: 3'- gGUAGGCCUGGUacaCCu----UUC-CCGCC- -5' |
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| 32835 | 3' | -54.7 | NC_007346.1 | + | 342999 | 0.75 | 0.796424 |
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Target: 5'- -aAUCCGGACgAUcUGGGAGGGGUGa -3' miRNA: 3'- ggUAGGCCUGgUAcACCUUUCCCGCc -5' |
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| 32835 | 3' | -54.7 | NC_007346.1 | + | 277547 | 0.76 | 0.76186 |
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Target: 5'- gUAUCCGGACCAgGUaGGAGAuuuuccccGGGUGGa -3' miRNA: 3'- gGUAGGCCUGGUaCA-CCUUU--------CCCGCC- -5' |
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| 32835 | 3' | -54.7 | NC_007346.1 | + | 315574 | 1.12 | 0.008639 |
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Target: 5'- uCCAUCCGGACCAUGUGGAAAGGGCGGu -3' miRNA: 3'- -GGUAGGCCUGGUACACCUUUCCCGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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